Water Extract of Yamato Tachibana (Citrus tachibana) Induces Food Intake in Adult and Larval Zebrafish
Water Extract of Yamato Tachibana (Citrus tachibana) Induces Food Intake in Adult and Larval Zebrafish
复制标题
大和橘（Citrus tachibana）的水提取物诱导成年斑马鱼和幼虫的食物摄入
DOI：
10.1089/jmf.2019.4484
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发表时间：
2020
影响因子：
2.4
通讯作者：
Shimada Yasuhito
中科院分区：
文献类型：
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作者：
Yamada Yuka;Chensom Sasicha;Yonemoto Hisataka;Nakayama Hiroko;Zang Liqing;Nishimura Norihiro;Mishima Takashi;Shimada Yasuhito
大和立花（立花;橘（Citrus tachibana）是一种特有水果，是日本最古老的柑橘品种之一;它生长在三重县。它因其文化遗产和独特的气味而备受关注。为了评估立花的生物活性，我们将立花果实的几个部分（整个果实，果肉[果肉和段壁]和外果皮）喂给成年斑马鱼，发现立花除了增加总体食物摄入量外，还增加了体重和血浆甘油三酯。然后，我们创建了一个简单的基于荧光的喂养试验，使用干燥的轮虫片和斑马鱼幼虫（受精后6天）筛选立花部分的各种提取物。我们发现立花果肉的水提取物增加了斑马鱼的摄食量。虽然柑橘类被认为可以预防肥胖和肥胖相关疾病，但我们的研究结果表明，橘的水提取物增加了斑马鱼的食物摄入量，并导致体重增加。我们认为，立花可能会扭转食欲不振的瘦人群，并可能证明有利于帮助鱼类养殖。
Yamato Tachibana (Tachibana;Citrus tachibana) is an endemic fruit and represents one of the oldest citrus species in Japan; it is grown in the Mie Prefecture. It has been attracting attention for its cultural heritage and unique scent. To evaluate biological activities of Tachibana, we fed several parts of the Tachibana fruit (whole fruit, pulp [albedo and segment wall], and flavedo) to adult zebrafish and found that Tachibana increased body weight and plasma triglycerides besides increasing overall food intake. We then created a simple fluorescence-based feeding assay using dried rotifer sheets and larval zebrafish (6 days postfertilization) to screen the various extracts of Tachibana parts. We found that water extracts of Tachibana pulp increased feeding volume in zebrafish. Although citrus species are believed to prevent obesity and obesity-associated diseases in general, our findings showed that water extracts of Tachibana increase food intake in zebrafish and lead to an increase in body weight. We suggest that Tachibana might reverse appetite loss in lean populations and may prove beneficial in aiding fish cultivation.