A80915, A NEW ANTIBIOTIC COMPLEX PRODUCED BY STREPTOMYCES-ACULEOLATUS - DISCOVERY, TAXONOMY, FERMENTATION, ISOLATION, CHARACTERIZATION, AND ANTIBACTERIAL EVALUATION
A80915, A NEW ANTIBIOTIC COMPLEX PRODUCED BY STREPTOMYCES-ACULEOLATUS - DISCOVERY, TAXONOMY, FERMENTATION, ISOLATION, CHARACTERIZATION, AND ANTIBACTERIAL EVALUATION
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DOI：
10.7164/antibiotics.43.623
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发表时间：
1990-06-01
影响因子：
3.3
通讯作者：
HOBBS, JN
中科院分区：
文献类型：
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作者：
FUKUDA, DS;MYNDERSE, JS;HOBBS, JN
从棘孢链霉菌（Streptomyces aculeolatus）A80915（NRRL 18422）发酵液中分离得到4个新的半萘醌类抗生素A80915 A、B、C和D。通过用乙酸乙酯提取回收存在于发酵液滤液和菌丝体甲醇提取物中的因子A和C以及主要存在于发酵液滤液中的因子B和D。通过制备型反相高效液相色谱法在C18键合的二氧化硅载体上完成各个因子的纯化。因子A D在体外对革兰氏阳性需氧菌和厌氧菌显示出抗菌活性。作用机制的研究表明，几乎完全抑制大分子生物合成（蛋白质，RNA，DNA和细胞壁）的A80915因子A.apprx。D.一个不太高的环化半萘醌，A80915因子G，分离自该菌株在交替培养基中发酵的肉汤。
New semi-naphthaquinone antibiotics A80915A, B, C, and D were isolated from the fermented broth of Streptomyces aculeolatus A80915 (NRRL 18422). Factors A and C, present in both the broth filtrate and mycelial methanol extract, and factors B and D, found predominantly in the broth filtrate, were recovered by extraction with ethyl acetate. Purification of the individual factors was accomplished by preparative reverse phase high performance liquid chromatography on C18 bonded silica supports. Factors A .apprx. D show antimicrobial activity against Gram-positive aerobic and anaerobic organisms in vitro. Mechanism of action studies demonstrated nearly complete inhibition of macromolecular biosynthesis (protein, RNA, DNA, and cell wall) by A80915 factors A .apprx. D. A less high cyclized semi-naphthaquinone, A80915 factor G, was isolated from the broth of the strain fermented in an alternate medium.