Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation
Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation
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DOI：
10.1364/oe.17.012502
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发表时间：
2009-07-20
期刊：
影响因子：
3.8
通讯作者：
Demos, Stavros G.
中科院分区：
文献类型：
--
作者：
Lin, Bevin;Urayama, Shiro;Demos, Stavros G.
在目前的临床实践中，食管疾病的检测仅限于肉眼可见的上皮形态学。在这项工作中，我们研究了高分辨率自发荧光成像下的紫外线激发可视化微观上皮细胞的变化与疾病的进展，使用台式原型显微镜。该方法是基于这样的假设，即UV激发光只能穿透细胞的表面层，从而产生上皮层的自发荧光图像，而不使用额外的图像切片方法。使用离体人食管活检标本进行实验。结果表明，与疾病进展相关的细胞形态学信息无需组织制备即可获得。(C)2009年美国光学学会
Detection of esophageal disease in current clinical practice is limited to visualization of macroscopic epithelial morphology. In this work, we investigate high resolution autofluorescence imaging under ultra violet excitation to visualize microscopic epithelial changes related to disease progression using a bench top prototype microscope. The approach is based on the hypothesis that UV excitation light can only penetrate the superficial layer of cells resulting in autofluorescence images of the epithelial layer without using an additional image sectioning approach. The experiments were performed using ex vivo human esophagus biopsy specimens. The results indicate that cellular morphology information related to disease progression is attainable without tissue preparation. (C) 2009 Optical Society of America