Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase.
Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase.
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DOI：
10.1038/ncomms15774
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发表时间：
2017-06-06
影响因子：
16.6
通讯作者：
Zenkin N
中科院分区：
文献类型：
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作者：
Forrest D;James K;Yuzenkova Y;Zenkin N
所有生物体中的转录都是通过多亚基RNA聚合酶（msRNAP）完成的。msRNAP在进化中是高度保守的，并且总是共享约400 kDa的五亚基催化核心。在这里，我们描述了一个假设的约100 kDa的单链蛋白，YonO，由枯草芽孢杆菌的SPβ原噬菌体编码。YonO与msRNAP具有非常远的同源性，但与单亚基聚合酶没有同源性。我们发现，尽管同源性只有少数氨基酸的msRNAP，和大多数的保守结构域的情况下，YonO是一个高度进行性的DNA依赖的RNA聚合酶。我们证明YonO是一个真正的RNAP的SPβ噬菌体，特异性转录其晚期基因，因此代表了一种新型的噬菌体RNAP。存在于各种细菌和噬菌体中的YonO和相关蛋白质在最后的通用共同祖先之前已经从msRNAP中分化出来，因此可能类似于所有msRNAP的单亚基祖先。虽然所有已知的RNA聚合酶都有多个亚基，但也有不相关的单亚基聚合酶。在这里，作者描述了来自枯草芽孢杆菌SPβ前噬菌体的单亚基RNA聚合酶，其与多亚基酶具有同源性。
Transcription in all living organisms is accomplished by multi-subunit RNA polymerases (msRNAPs). msRNAPs are highly conserved in evolution and invariably share a ∼400 kDa five-subunit catalytic core. Here we characterize a hypothetical ∼100 kDa single-chain protein, YonO, encoded by the SPβ prophage of Bacillus subtilis. YonO shares very distant homology with msRNAPs, but no homology with single-subunit polymerases. We show that despite homology to only a few amino acids of msRNAP, and the absence of most of the conserved domains, YonO is a highly processive DNA-dependent RNA polymerase. We demonstrate that YonO is a bona fide RNAP of the SPβ bacteriophage that specifically transcribes its late genes, and thus represents a novel type of bacteriophage RNAPs. YonO and related proteins present in various bacteria and bacteriophages have diverged from msRNAPs before the Last Universal Common Ancestor, and, thus, may resemble the single-subunit ancestor of all msRNAPs. Although all known RNA polymerases have multiple subunits, unrelated single-subunit polymerases have also been described. Here, the authors describe a single-subunit RNA polymerase from the SPβ prophage of Bacillus subtilis, which shares homology to multi-subunit enzymes.