An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp PCC6803
An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp PCC6803
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DOI：
10.1073/pnas.1015154108
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发表时间：
2011-02-01
影响因子：
11.1
通讯作者：
Hess, Wolfgang R.
中科院分区：
文献类型：
--
作者：
Mitschke, Jan;Georg, Jens;Hess, Wolfgang R.
人们对蓝藻的兴趣越来越大，因为这些光合生物将太阳能转化为生物质，因为它们有生产生物燃料的潜力。然而，蓝藻生物工程的开发需要他们的转录组织的知识。利用差分RNA测序技术，我们建立了模式生物聚囊藻PCC6803的3527个转录起始位点（TSS）的全基因组图谱。三分之一的TSS位于被注释基因的上游；另外三分之一位于866个基因的反向互补链上，表明大量的反义转录。位于基因间区域的孤儿TSS使我们预测了314种非编码rna （ncRNAs）。基于互补微阵列的RNA分析验证了大量的非编码转录本，并确定了强大的ncRNA调控。因此，大约64%的TSS在基因组中产生反义或ncrna，这占蛋白质编码的87%。我们的数据将启动子的信息增强了40倍，表明存在额外的小肽编码mrna，并为该蓝藻的许多基因提供了校正的5'注释。全球TSS图谱将有助于将Synechocystis sp. PCC6803作为模式生物进行进一步的光合作用和能量研究。
There has been an increasing interest in cyanobacteria because these photosynthetic organisms convert solar energy into biomass and because of their potential for the production of biofuels. However, the exploitation of cyanobacteria for bioengineering requires knowledge of their transcriptional organization. Using differential RNA sequencing, we have established a genome-wide map of 3,527 transcriptional start sites (TSS) of the model organism Synechocystis sp. PCC6803. One-third of all TSS were located upstream of an annotated gene; another third were on the reverse complementary strand of 866 genes, suggesting massive antisense transcription. Orphan TSS located in intergenic regions led us to predict 314 noncoding RNAs (ncRNAs). Complementary microarray-based RNA profiling verified a high number of noncoding transcripts and identified strong ncRNA regulations. Thus, similar to 64% of all TSS give rise to antisense or ncRNAs in a genome that is to 87% protein coding. Our data enhance the information on promoters by a factor of 40, suggest the existence of additional small peptide-encoding mRNAs, and provide corrected 5' annotations for many genes of this cyanobacterium. The global TSS map will facilitate the use of Synechocystis sp. PCC6803 as a model organism for further research on photosynthesis and energy research.