PREHEPATIC INSULIN PRODUCTION IN MAN - KINETIC-ANALYSIS USING PERIPHERAL CONNECTING PEPTIDE BEHAVIOR
PREHEPATIC INSULIN PRODUCTION IN MAN - KINETIC-ANALYSIS USING PERIPHERAL CONNECTING PEPTIDE BEHAVIOR
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DOI：
10.1210/jcem-51-3-520
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发表时间：
1980-01-01
影响因子：
5.8
通讯作者：
STANDEFER, J
中科院分区：
文献类型：
--
作者：
EATON, RP;ALLEN, RC;STANDEFER, J
通过对男性和女性血浆中连接肽（C肽）行为的动力学分析，评价了正常男性肝前胰岛素的产生。在内源性胰岛素分泌抑制期间（通过空腹和外源性胰岛素注射诱导）以及刺激分泌期间（通过口服葡萄糖摄入诱导）和静脉注射葡萄糖期间进行研究。通过交互式计算机分析的C肽数据的最小二乘样条拟合允许使用用于C肽去除的2室模型评价胰岛素的前体生产。基础肝前胰岛素产生平均为15 ± 1.5。在20名受试者中为4 mU[单位]/70 kg/min，并降低至0.9 ±。2.2禁食84小时后，mU/70 kg/min。注射外源性静脉内胰岛素导致内源性产生抑制至0 . ± 0. 2.5由100-g口服葡萄糖耐量试验诱导的最大肝前胰岛素产生量为91 ± 0.5mU/70 kg/min。1.2 mU/70 kg/min，累积激素分泌为11.4 ±。2.0在5小时的观察中。在急性静脉注射25 g葡萄糖后，产量上升至465 ± 0.001。在注射后2分钟时为108 mU/70 kg/min，并迅速恢复至基础水平。相对于同时的外周静脉水平，根据该分析计算的门静脉胰岛素水平显着升高。这些结果定量肝前胰岛素的生产和门静脉胰岛素浓度从分析的C-肽的行为在血浆中的稳态和非稳态在人。
The prehepatic production of insulin in normal man was evaluated by kinetic analysis of connecting peptide (C-peptide) behavior in the plasma in men and women. Studies were performed during suppression of endogenous insulin secretion (induced by both fasting and exogenous insulin injection) as well as during stimulation of secretion (induced by oral glucose ingestion) and i.v. glucose injection. Least squares spline fitting of the C-peptide data by interactive computer analysis permitted evaluation of the precursor production of insulin using a 2-compartment model for C-peptide removal. Basal prehepatic insulin production averaged 15 .+-. 4 mU[units]/70 kg per min in 20 subjects and was reduced to 0.9 .+-. 2.2 mU/70 kg per min after 84 h of fasting. The injection of exogenous i.v. insulin resulted in suppression of endogenous production to 0 .+-. 2.5 mU/70 kg per min. Maximum prehepatic insulin production induced by a 100-g oral glucose tolerance test was 91 .+-. 1.2 mU/70 kg per min, with a cumulative hormone secretion of 11.4 .+-. 2.0 U over the 5 h of observation. After the acute i.v. injection of 25 g glucose, production rose to 465 .+-. 108 mU/70 kg per min at 2 min post injection and rapidly returned toward basal. Levels of insulin in the portal vein calculated from this analysis were markedly elevated relative to simultaneous peripheral venous levels. These results quantitate prehepatic insulin production and portal venous insulin concentration from an analysis of the behavior of C-peptide within the plasma in both the steady state and the nonsteady state in man.