The Streptococcus faecalis oxidases for reduced diphosphopyridine nucleotide. III. Isolation and properties of a flavin peroxidase for reduced diphosphopyridine nucleotide.

The Streptococcus faecalis oxidases for reduced diphosphopyridine nucleotide. III. Isolation and properties of a flavin peroxidase for reduced diphosphopyridine nucleotide.
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粪链球菌氧化酶可还原二磷酸吡啶核苷酸。

DOI:
10.1016/s0021-9258(18)64952-x
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发表时间:
1957
期刊:
The Journal of biological chemistry
影响因子:
--
通讯作者:
M. Dolin
M. Dolin
中科院分区:
--
文献类型:
--
作者:
M. Dolin

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方法淀粉块区带电泳-Kunkel和斯莱特(5)指出了淀粉作为支持介质的优点。基本上使用了Rotman和Spiegelman(6)的技术,只是规定了淀粉块的液体冷却和在橡树岭国家实验室根据原子能委员会的合同号W-7405-Eng-26进行的返工。1本文中使用的缩写是DPNH,还原的二磷酸吡啶核苷酸; DPN,二磷酸吡啶核苷酸; FAD,黄素腺嘌呤二核苷酸; BAL,2,3-二巯基丙醇; TPNH,还原的三磷酸吡啶核苷酸; FP,黄素蛋白; TPN,三磷酸吡啶核苷酸。
MethodsZone Electrophoresis on Starch Blocks-The advantages of starch as a supporting medium have been pointed out by Kunkel and Slater (5). Essentially the technique of Rotman and Spiegelman (6) was used, except that provisions were made for liquid cooling of the starch block and for re-* Work performed at the Oak Ridge National Laborat, ory under contract No. W-7405-Eng-26 for the Atomic Energy Commission. 1 The abbreviations used in this paper are DPNH, reduced diphosphopyridine nucleotide; DPN, diphosphopyridine nucleotide; FAD, flavin adenine dinucleotide; BAL, 2, 3-dimercaptopropanol; TPNH, reduced triphosphopyridine nucleotide; FP, flavoprotein; TPN, triphosphopyridine nucleotide.