Stanniocalcin-2 (STC2): A potential lung cancer biomarker promotes lung cancer metastasis and progression
Stanniocalcin-2 (STC2): A potential lung cancer biomarker promotes lung cancer metastasis and progression
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DOI：
10.1016/j.bbapap.2014.11.002
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发表时间：
2015-06-01
影响因子：
3.2
通讯作者：
Cho, Je-Yoel
中科院分区：
文献类型：
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作者：
Na, Sang-su;Aldonza, Mark Borris;Cho, Je-Yoel
同型二聚体糖蛋白stanniocalcin 2 （STC2）先前已知参与肾脏中钙和磷酸盐运输的调节，也报道在几种癌症中发挥多种作用。然而，其在肺癌中的作用和临床意义尚未见报道，仍不确定。在这里，我们研究了STC2作为肺癌生物标志物的可能性，并确定了其在肺癌细胞生长、转移和进展中的潜在作用。原代培养的肺癌细胞分泌组的蛋白质组学分析显示，与邻近正常细胞相比，癌细胞中STC2的表达更高。RT-PCR和Western blot分析显示，肺癌组织中STC2 mRNA和蛋白的表达高于癌旁正常组织。在H460肺癌细胞中，STC2的敲低可减缓细胞生长进程和集落形成。进一步的分析显示，STC2敲低细胞的迁移、侵袭和G(0)/G(1)细胞周期的延迟受到抑制。STC2敲低还能减弱H(2)0(2)诱导的氧化应激对H460细胞活力的影响，并随之增加细胞内ROS水平，提示STC2在肺癌氧化还原调控系统中具有保护作用。这些发现提示STC2可能是一种潜在的肺癌生物标志物，在肺癌转移和进展中发挥积极作用。本文是《医学蛋白质组学》特刊的一部分。(C) 2015 Elsevier B.V.版权所有
The homodimeric glycoprotein, stanniocalcin 2 (STC2) is previously known to be involved in the regulation of calcium and phosphate transport in the kidney and also reported to play multiple roles in several cancers. However, its function and clinical significance in lung cancer have never been reported and still remain uncertain. Here, we investigated the possibility of STC2 as a lung cancer biomarker and identified its potential role in lung cancer cell growth, metastasis and progression. Proteomic analysis of secretome of primary cultured lung cancer cells revealed higher expression of STC2 in cancers compared to that of adjacent normal cells. RT-PCR and Western blot analyses showed higher mRNA and protein expressions of STC2 in lung cancer tissues compared to the adjacent normal tissues. Knockdown of STC2 in H460 lung cancer cells slowed down cell growth progression and colony formation. Further analysis revealed suppression of migration, invasion and delayed G(0)/G(1) cell cycle progression in the STC2 knockdown cells. STC2 knockdown also attenuated the H(2)0(2)-induced oxidative stress on H460 cell viability with a subsequent increase in intracellular ROS levels, which suggest a protective role of STC2 in redox regulatory system of lung cancer. These findings suggest that STC2 can be a potential lung cancer biomarker and plays a positive role in lung cancer metastasis and progression. This article is part of a Special Issue entitled: Medical Proteomics. (C) 2015 Elsevier B.V. All rights reserved.