Inhibitory effects of patchouli alcohol on stress-induced diarrhea-predominant irritable bowel syndrome.
Inhibitory effects of patchouli alcohol on stress-induced diarrhea-predominant irritable bowel syndrome.
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广藿香醇对应激性腹泻型肠易激综合征的抑制作用
DOI：
10.3748/wjg.v24.i6.693
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发表时间：
2018-02-14
影响因子：
4.3
通讯作者：
Tan B
中科院分区：
文献类型：
--
作者：
Zhou TR;Huang JJ;Huang ZT;Cao HY;Tan B
目的：探讨广藿香醇(PA)治疗腹泻型肠易激综合征(IBS-D)大鼠模型的作用机制。用PA的累积对数浓度(3×10−7m ol/L至1×10−4m o l/L)研究PA对结肠自发运动的影响。(2)神经递质拮抗剂N-硝基-L-精氨酸甲酯盐酸盐(N-2S*)-4-[2-Iodo-6-(methylamino)-9H-purin-9-yl]-2-(phosphonooxy)bicyclo[3.1.0]hexane-1-methanol-硝基-L-精氨酸甲酯盐酸盐)(10−/L)和(1R*，−磷酸二氢酯四铵盐(1μ/L))；(3)激动剂α，β-亚甲基腺苷5‘-三磷酸三钠(100mmoL/L)；(4)单剂量KCl120μ/L。用PA(100μ摩尔/L)预处理1min，观察阻断剂对拮抗剂反应的影响。采用40V，2~30 Hz，0.5ms脉冲宽度，10 S的电场刺激，观察IBS-D大鼠结肠非肾上腺素能、非胆碱能神经递质的释放。同时测定Kreb‘s液的ATP水平。PA对结肠纵行肌自发性收缩的抑制作用呈浓度依赖性，半数最大有效浓度(EC50)为41.9Mol/L。与KCl组比较，PA+KClIBS-D组的收缩反应(mg收缩)在峰值张力时显著降低(11.87±3.34)(P<；0.01)。与CCH处理的IBS-D大鼠相比，PA能显著降低IBS-D大鼠结肠的胆碱能收缩反应(EC_(50)=0.94Mol/L)(EC_(50)=37.43μμ/L)(P<；0.05)。应激性IBS-D大鼠氮能神经递质释放不足对结肠平滑肌有收缩作用。PA可明显抑制L(10μ/L)引起的心肌细胞收缩。三磷酸腺苷可能不是PA抑制应激性IBS-D大鼠结肠松弛的主要神经递质。PA敷贴有望成为治疗IBS-D的一种新方法。
To elucidate the mechanism of patchouli alcohol (PA) in treatment of rat models of diarrhea-predominant irritable bowel syndrome (IBS-D). We studied the effects of PA on colonic spontaneous motility using its cumulative log concentration (3 × 10−7 mol/L to 1 × 10−4 mol/L). We then determined the responses of the proximal and distal colon segments of rats to the following stimuli: (1) carbachol (1 × 10−9 mol/L to 1 × 10−5 mol/L); (2) neurotransmitter antagonists including Nω-nitro-L-arginine methyl ester hydrochloride (10 μmol/L) and (1R*, 2S*)-4-[2-Iodo-6-(methylamino)-9H-purin-9-yl]-2-(phosphonooxy)bicyclo[3.1.0]hexane-1-methanol dihydrogen phosphate ester tetraammonium salt (1 μmol/L); (3) agonist α,β-methyleneadenosine 5′-triphosphate trisodium salt (100 μmol/L); and (4) single KCl doses (120 mmol/L). The effects of blockers against antagonist responses were also assessed by pretreatment with PA (100 μmol/L) for 1 min. Electrical-field stimulation (40 V, 2-30 Hz, 0.5 ms pulse duration, and 10 s) was performed to observe nonadrenergic, noncholinergic neurotransmitter release in IBS-D rat colon. The ATP level of Kreb’s solution was also determined. PA exerted a concentration-dependent inhibitory effect on the spontaneous contraction of the colonic longitudinal smooth muscle, and the half maximal effective concentration (EC50) was 41.9 μmol/L. In comparison with the KCl-treated IBS-D group, the contractile response (mg contractions) in the PA + KCl-treated IBS-D group (11.87 ± 3.34) was significantly decreased in the peak tension (P < 0.01). Compared with CCh-treated IBS-D rat colon, the cholinergic contractile response of IBS-D rat colonic smooth muscle (EC50 = 0.94 μmol/L) was significantly decreased by PA (EC50 = 37.43 μmol/L) (P < 0.05). Lack of nitrergic neurotransmitter release in stress-induced IBS-D rats showed contraction effects on colonic smooth muscle. Pretreatment with PA resulted in inhibitory effect on L-NAME-induced (10 μmol/L) contraction (P < 0.05). ATP might not be the main neurotransmitter involved in inhibitory effects of PA in the colonic relaxation of stress-induced IBS-D rats. PA application may serve as a new therapeutic approach for IBS-D.
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影响因子：
2.9
作者：
Callahan, MJ
通讯作者：
Callahan, MJ
影响因子：
3.5
作者：
Manabe N;Wong BS;Camilleri M;Burton D;McKinzie S;Zinsmeister AR
通讯作者：
Zinsmeister AR
影响因子：
6.3
作者：
Hafting, T.;Haug, T. M.;Sand, O.
通讯作者：
Sand, O.
影响因子：
3.3
作者：
Peiris M;Hockley JR;Reed DE;Smith ESJ;Bulmer DC;Blackshaw LA
通讯作者：
Blackshaw LA
DOI：
10.1080/17474124.2017.1298442
发表时间：
2017-01-01
影响因子：
3.9
作者：
Corsetti, Maura;Whorwell, Peter
通讯作者：
Whorwell, Peter