Anti-tumor effect of orally administered spinach glycolipid fraction on implanted cancer cells, colon-26, in mice
Anti-tumor effect of orally administered spinach glycolipid fraction on implanted cancer cells, colon-26, in mice
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DOI：
10.1007/s11745-008-3202-5
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发表时间：
2008-08-01
期刊：
影响因子：
1.9
通讯作者：
Mizushina, Yoshiyuki
中科院分区：
文献类型：
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作者：
Maeda, Naoki;Kokai, Yasuo;Mizushina, Yoshiyuki
我们成功地从绿色蔬菜菠菜中提纯了一种主要的糖脂部分。这一组分主要由三种糖脂组成：单半乳糖二酰甘油(MGDG)、双半乳糖二酰甘油(DGDG)和磺基喹诺酮二甘油(SQDG)。在之前的研究中，我们发现糖脂部分通过皮下注射抑制DNA聚合酶活性、癌细胞生长和肿瘤生长。我们的目的是阐明口服糖脂部分，抑制小鼠结肠腺癌(结肠-26)肿瘤生长的作用。一项肿瘤移植研究表明，口服20 mg/kg糖脂组分2周后，小鼠实体瘤体积减少了56.1%(P<；0.05)，且没有任何副作用，如体重减轻或主要器官衰竭。糖脂组分通过抑制肿瘤血管生成和肿瘤组织中细胞增殖标记物Ki-67、增殖细胞核抗原和细胞周期蛋白E的表达而抑制肿瘤生长。这些结果表明，口服菠菜糖脂部分可通过抑制肿瘤组织中新生血管的活性和癌细胞的增殖而抑制小鼠结肠癌的生长。
We succeeded in purifying a major glycolipid fraction from a green vegetable, spinach. This fraction consists mainly of three glycolipids: monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), and sulfoquinovosyl diacylglycerol (SQDG). In a previous study, we found that the glycolipid fraction inhibited DNA polymerase activity, cancer cell growth and tumor growth with subcutaneous injection. We aimed to clarify oral administration of the glycolipid fraction, suppressing colon adenocarcinoma (colon-26) tumor growth in mice. A tumor graft study showed that oral administration of 20 mg/kg glycolipid fraction for 2 weeks induced a 56.1% decrease in the solid tumor volume (P < 0.05) without any side-effects, such as loss of body weight or major organ failure, in mice. The glycolipid fraction induced the suppression of colon-26 tumor growth with inhibition of angiogenesis and the expression of cell proliferation marker proteins such as Ki-67, proliferating cell nuclear antigen (PCNA), and Cyclin E in the tumor tissue. These results suggest that the orally administered glycolipid fraction from spinach could suppress colon tumor growth in mice by inhibiting the activities of neovascularization and cancer cellular proliferation in tumor tissue.