Immunolocalization of type IV collagen and laminin in nonbasement membrane structures of murine corneal stroma. A light and electron microscopic study.
Immunolocalization of type IV collagen and laminin in nonbasement membrane structures of murine corneal stroma. A light and electron microscopic study.
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发表时间：
1985-06
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通讯作者：
B. Pratt;J. Madri
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作者：
B. Pratt;J. Madri
成体脊椎动物角膜基质的细胞外基质主要由间质的I型胶原和少量的III型和V型胶原组成。这些胶原蛋白被组织成由条纹细丝组成的重叠片层。除了这些板层结构，角膜基质还包含100到250纳米的无条纹8到11纳米的微纤维束。通过免疫荧光定位和电子显微镜免疫定位，小鼠体内的这些微纤维束与III型胶原、IV型胶原和层粘连蛋白有关。根据免疫学和组织化学标准，这些纤维束既不包含I型胶原，也不包含V型胶原，不包含弹性蛋白，也不包含奥他兰微纤维。讨论了这些微原纤束的细胞来源、组成和可能的功能。
The extracellular matrix of adult vertebrate corneal stroma is composed primarily of the interstitial collagen type I and smaller amounts of types III and V collagen. These collagens are organized into overlapping lamellae of striated filaments. In addition to these lamellar structures, the corneal stroma also contains 100- to 250-nm bundles of nonstriated 8- to 11-nm microfibrils. By immunofluorescent localization and electron microscopic immunolocalization, these microfibril bundles in the mouse are associated with type III collagen, type IV collagen, and laminin. By immunologic and histochemical criteria, these bundles do not contain either type I collagen, type V collagen, elastin, or oxytalan microfibrils. The cellular source, composition, and possible functions of these microfibril bundles are discussed.