CYP81A68 confers metabolic resistance to ALS and ACCase-inhibiting herbicides and its epigenetic regulation in Echinochloa crus-galli
CYP81A68 confers metabolic resistance to ALS and ACCase-inhibiting herbicides and its epigenetic regulation in Echinochloa crus-galli
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CYP81A68赋予稗草对ALS和ACCase抑制性除草剂的代谢抗性及其表观遗传调控
DOI：
10.1016/j.jhazmat.2022.128225
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发表时间：
2022-01-12
影响因子：
13.6
通讯作者：
Bai, Lianyang
中科院分区：
文献类型：
--
作者：
Pan, Lang;Guo, Qiushuang;Bai, Lianyang
长期过量使用除草剂已引起环境问题，特别是杂草的除草剂抗性进化。在这里，我们证实了乙酰乳酸合酶（ALS）抑制除草剂五氟磺草胺抗性和对乙酰辅酶羧化酶（ACCase）抑制除草剂（氰氟草酯和吡禾灵）的交叉抗性，在全球杂草稗草种群中对这些除草剂具有抗性（R）。五氟磺草胺在R中的降解速率显著高于敏感E. crus-galli种群（S）。RNA测序显示细胞色素P450（P450）基因CYP 81 A68在R中的表达高于S。过量表达该CYP 81 A68基因的水稻幼苗对五氟磺草胺、氰氟草酯和吡禾灵具有抗性，而五氟磺草胺抗性是由于通过O-去甲基化增强代谢所致。对CYP 81 A68基因启动子的缺失分析确定了一个有效区域，在该区域R和S之间发生了CpG岛的差异甲基化。总的来说，这些结果表明，上调E。稗草CYP 81 A68基因赋予水稻对ALS和ACCase抑制型除草剂的多能代谢抗性，表观遗传调控可能在抗性进化中发挥作用。本研究可为合理选择除草剂替代品和采取非化学防治策略减少除草剂对环境的影响提供参考。
Long-term and excessive herbicide use has led to some environmental concerns and especially, herbicide resistance evolution in weeds. Here, we confirmed acetolactate synthase (ALS) inhibiting herbicide penoxsulam resistance and cross resistance to acetyl-coenzyme carboxylase (ACCase) inhibiting herbicides (cyhalofop-butyl and metamifop) in a global weed Echinochloa crus-galli population resistant to these herbicides (R). Penoxsulam metabolism study indicated that degradation rate was significantly higher in R than susceptible E. crus-galli population (S). RNA-sequencing revealed that a cytochrome P450 (P450) gene, CYP81A68, expressed higher in R versus S. Rice seedlings overexpressing this CYP81A68 gene are resistant to penoxsulam, cyhalofop-butyl and metamifop, and penoxsulam resistance is due to enhanced metabolism via O-demethylation. Deletion analysis of the CYP81A68 gene promoter identified an efficient region, in which differential methylation of CpG islands occurred between R and S. Collectively, these results demonstrate that upregulation of E. crus-galli CYP81A68 gene endows generalist metabolic resistance to commonly used ALS- and ACCase-inhibiting herbicides in rice fields and epigenetic regulation may play a role in the resistance evolution. This research could contribute to strategies reducing herbicide environmental impacts by judicious selection of alternative herbicide and nonchemical control tactics.