The positive and negative transcriptional regulation of the Drosophila Gapdh-2 gene.
The positive and negative transcriptional regulation of the Drosophila Gapdh-2 gene.
复制标题
果蝇 Gapdh-2 基因的正向和负向转录调控。
DOI：
10.1101/gad.2.6.743
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发表时间：
1988
影响因子：
10.5
通讯作者：
Wu,R
中科院分区：
文献类型：
--
作者：
Sun,XH;Lis,JT;Wu,R
编码黑腹果蝇甘油醛3-磷酸脱氢酶的基因之一Gapdh-2在所有检测的细胞类型中表达，但其表达水平受发育调节。在这里，我们报告的Gapdh-2的转录调控序列的分析。我们已经产生了Gapdh-2-LacZ融合基因，其中Gapdh-2的5 '侧翼序列已经突变。通过转染引入Schneider II细胞系和通过种系转化引入果蝇，对这些融合基因的表达进行检测，在Gapdh-2的5 ′-侧翼序列的前145 bp内鉴定出两个不同的调节区URS-1和URS-2。URS-1在果蝇的整个发育过程中激活转录。然而，URS-2在开发过程中表现出双重功能。它明显抑制Schneider II细胞的转录，可能也抑制中期胚胎的转录。相反，它激活幼虫和成年苍蝇的转录。因此，URS-2在Gapdh-2的发育调节中起关键作用。此外，Gapdh-2在幼虫和成虫阶段的有效转录似乎取决于URS-1和URS-2的协同功能。
One of the genes encoding glyceraldehyde 3-phosphate dehydrogenase of Drosophila melanogaster, Gapdh-2, is expressed in all cell types examined, but its level of expression is regulated developmentally. Here we report the analysis of the regulatory sequences for the transcription of Gapdh-2. We have generated Gapdh-2-LacZ fusion genes in which the 5'-flanking sequence of Gapdh-2 has been mutated. Examination of the expression of these fusion genes, which have been introduced by transfection into the Schneider II cell line and by germ-line transformation into flies, led to the identification of two distinct regulatory regions, URS-1 and URS-2, within the first 145 bp of the 5'-flanking sequence of Gapdh-2. URS-1 activates transcription throughout the development of Drosophila. However, URS-2 exhibits a dual function during the development. It clearly represses transcription in Schneider II cells, and perhaps also in mid-stage embryos. In contrast, it activates transcription in larvae and adult flies. Thus, URS-2 plays a key role in the developmental regulation of Gapdh-2. Additionally, the efficient transcription of Gapdh-2 in larval and adult stages appears to depend on a synergistic function of URS-1 and URS-2.
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阻遏物（MATα2 产品）及其操纵子控制酵母中一组细胞类型特异性基因的表达
影响因子：
64.5
作者：
A. Johnson;I. Herskowitz
通讯作者：
I. Herskowitz
两个不连锁的黑腹果蝇甘油醛-3-磷酸脱氢酶基因的结构。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tso,JY;Sun,XH;Wu,R
通讯作者：
Wu,R
用 hsp70-lacZ 杂合基因转化果蝇产生的新热休克泡芙和 β=半乳糖苷酶活性
影响因子：
64.5
作者：
J. Lis;J. Simon;C. Sutton
通讯作者：
C. Sutton
DOI：
--
发表时间：
1983
影响因子：
11.1
作者：
P. P. D. Nocera;Igor B. Dawid
通讯作者：
Igor B. Dawid
DOI：
10.1073/pnas.83.16.5889
发表时间：
1986-08-01
影响因子：
11.1
作者：
KADONAGA, JT;TJIAN, R
通讯作者：
TJIAN, R