Detection of Equid Herpesvirus 9 DNA in the Trigeminal Ganglia of a Burchell's Zebra from the Serengeti Ecosystem
Detection of Equid Herpesvirus 9 DNA in the Trigeminal Ganglia of a Burchell's Zebra from the Serengeti Ecosystem
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DOI：
10.1292/jvms.70.1377
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发表时间：
2008-12-01
影响因子：
1.2
通讯作者：
Hoare, Richard
中科院分区：
文献类型：
--
作者：
Borchers, Kerstin;Lieckfeldt, Dietmar;Hoare, Richard
马疱疹病毒9(EHV-9)是从一群受脑炎影响的汤姆森瞪羚中分离出来的。EHV-9的自然宿主尚不清楚，但斑马被怀疑是瞪羚的感染源。为了证明这一假设，我们分析了43份来自伯切尔斑马(Equus Burchelli)和21份来自塞伦盖蒂生态系统的汤姆森瞪羚(Gazella Thomsoni)的血清，以寻找中和抗体。从GREVY斑马株T965和T616中检出7份EHV-1、EHV-9和EHV-1阳性斑马血清。用EHV-9 gB和EHV-1 ICPO特异性套式聚合酶链式反应(PCR)对另外17头Burchell‘s斑马和1头Thomson’s瞪羚的三叉神经节进行检测。聚合酶链式反应测序证实其中一个斑马节为EHV-9阳性。这些结果表明，伯切尔斑马接触了EHV-9并潜伏感染。
Equid herpesvirus 9 (EHV-9) was isolated from a herd of Thomson's gazelles affected by encephalitis. The natural host of EHV-9 is unknown, but zebras are suspected to be the source of infection in gazelles. To prove this hypothesis, we analyzed 43 sera from Burchell's zebras (Equus burchelli) and 21 Thomson's gazelles (Gazella thomsoni) from the Serengeti ecosystem for neutralizing antibodies. Seven zebra sera were positive for EHV-1, EHV-9 and EHV-1 from Grevy's zebra strains T965 and T616. The trigeminal ganglia of 17 other Burchell's zebras and one Thomson's gazelle were tested by EHV-9 gB and EHV-1 ICPO-specific nested polymrase chain reaction (PCR). PCR Sequencing confirmed that one zebra ganglion was positive for EHV-9. These results Suggest that the Burchell's zebras were exposed to EHV-9 and latently infected.