Can prenatal n-3 fatty acid deficiency be completely reversed after birth? Effects on retinal and brain biochemistry and visual function in rhesus monkeys
Can prenatal n-3 fatty acid deficiency be completely reversed after birth? Effects on retinal and brain biochemistry and visual function in rhesus monkeys
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DOI：
10.1203/01.pdr.0000182188.31596.5a
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发表时间：
2005-11-01
影响因子：
3.6
通讯作者：
Connor, WE
中科院分区：
文献类型：
--
作者：
Anderson, GJ;Neuringer, M;Connor, WE
我们之前对恒河猴的研究表明，产前和产后联合缺乏n-3脂肪酸会导致视力下降，视网膜功能异常，视网膜和脑二十二碳六烯酸含量低。我们现在只报道了n-3脂肪酸缺乏对宫内发育的影响。恒河猴的母亲在整个怀孕期间都喂食缺乏n-3脂肪酸的饮食，从出生到3岁时喂食富含α -亚麻酸的饮食。在几个时间点测定血浆和红细胞的脂肪酸组成，在15、30、45和60周进行前额叶大脑皮层活检，在3岁时测定大脑皮层和视网膜的脂肪酸组成。在出生后4、8和12周测定视力。3-4月龄时记录视网膜电图。出生时血浆、红细胞和组织中总n-3脂肪酸减少70-90%，但在4周内血浆、8周内红细胞和15周内大脑皮层恢复到控制值。3岁时，脑磷脂脂肪酸组成正常，但视网膜DHA恢复不完全（占对照组的84%）。视觉敏锐度阈值与母亲喂养高亚麻酸饮食的对照组婴儿没有差异。然而，饱腹组锥体和杆状ERG - a波振幅较低。这些数据表明，在产前限制n-3脂肪酸的摄入可能对视网膜脂肪酸组成和功能有长期影响。
Our previous studies of rhesus monkeys showed that combined prenatal and postnatal n-3 fatty acid deficiency resulted in reduced visual acuity, abnormal retinal function, and low retina and brain docosahexaenoic acid content. We now report effects of n-3 fatty acid deficiency during intrauterine development only. Rhesus infants, born to mothers fed an n-3 fatty acid deficient diet throughout pregnancy, were repleted with a diet high in alpha-linolenic acid from birth to 3 y. Fatty acid composition was determined for plasma and erythrocytes at several time points, for prefrontal cerebral cortex biopsies at 15, 30, 45, and 60 wk, and for cerebral cortex and retina at 3 y. Visual acuity was determined behaviorally at 4, 8, and 12 postnatal weeks, and the electroretinogram was recorded at 3-4 mo. Total n-3 fatty acids were reduced by 70-90% in plasma, erythrocytes, and tissues at birth but recovered to control values within 4 wk in plasma, 8 wk in erythrocytes, and 15 wk in cerebral cortex. At 3 y, fatty acid composition was normal in brain phospholipids, but in the retina DHA recovery was incomplete (84% of controls). Visual acuity thresholds did not differ from those of control infants from mothers fed a high linolenic acid diet. However, the repleted group had lower amplitudes of cone and rod ERG a-waves. These data suggest that restriction of n-3 fatty acid intake during the prenatal period may have long-term effects on retinal fatty acid composition and function.