Immobilizing bacitracin on titanium for prophylaxis of infections and for improving osteoinductivity: An in vivo study
Immobilizing bacitracin on titanium for prophylaxis of infections and for improving osteoinductivity: An in vivo study
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将杆菌肽固定在钛上预防感染和改善骨诱导性：一项体内研究
DOI：
10.1016/j.colsurfb.2016.11.034
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发表时间：
2017-02-01
影响因子：
5.8
通讯作者：
Yue, Bing
中科院分区：
文献类型：
--
作者：
Nie, Bin'en;Ao, Haiyong;Yue, Bing
固定在钛（Ti）表面的杆菌肽显着提高了体外抗菌活性和生物相容性。在本研究中，我们研究了杆菌肽修饰的钛在体内的生物学性能（杀菌作用和骨-植入物整合）。采用股骨髓腔放置钛棒的大鼠骨髓炎模型来分析杆菌肽修饰钛（Ti-BC）的预防作用。三十六只雌性 Sprague Dawley (SD) 大鼠被用来建立钛植入物相关的感染。将Ti和Ti-BC棒与金黄色葡萄球菌一起孵育，以模拟污染的Ti棒，并将其植入左股骨髓腔中，并使用无菌Ti棒作为空白对照。 3周后，使用X射线和微型计算机断层扫描（micro-CT）分析评估骨病理学。为了研究 Ti-BC 种植体的体内骨整合，将 15 只 SD 大鼠分为三组 (N = 5)，即 Ti、Ti-多巴胺固定 (Ti-DOPA) 和 Ti-BC。将钛棒植入左侧股骨腔，12周后进行显微CT和组织学评估。体内研究表明，钛固定杆菌肽具有预防与钛种植体相关的感染的潜力，并允许骨整合。因此，多重生物功能化钛种植体可以通过固定杆菌肽来实现，使其成为预防钛种植体相关感染同时保留骨整合效应的有希望的候选者。 (C) 2016 Elsevier B.V. 保留所有权利。
Bacitracin immobilized on the titanium (Ti) surface significantly improves anti-bacterial activity and biocompatibility in vitro. In the current study, we investigated the biologic performance (bactericidal effect and bone-implant integration) of bacitracin-modified Ti in vivo. A rat osteomyelitis model with femoral medullary cavity placement of Ti rods was employed to analyze the prophylactic effect of bacitracin-modified Ti (Ti-BC). Thirty-six female Sprague Dawley (SD) rats were used to establish the Ti implant-associated infection. The Ti and Ti-BC rods were incubated with and without Staphylococcus aureus to mimic the contaminated Ti rod and were implanted into the medullary cavity of the left femur, and sterile Ti rods were used as the blank control. After 3 weeks, the bone pathology was evaluated using X-ray and micro-computed tomography (micro-CT) analysis. For the investigation of the Ti-BC implant osseointegration in vivo, fifteen SD rats were divided into three groups (N = 5), namely Ti, Ti-dopamine immobilized (Ti-DOPA), and Ti-BC. Ti rods were implanted into the left femoral cavity and micro-CT and histological evaluation was conducted after 12 weeks. The in vivo study indicated that Ti-immobilized bacitracin owned the prophylaxis potential for the infection associated with the Ti implants and allowed for the osseointegration. Thus, the multiple biofunctionalized Ti implants could be realized via immobilization of bacitracin, making them promising candidates for preventing the Ti implant-associated infections while retaining the osseointegration effects. (C) 2016 Elsevier B.V. All rights reserved.