Expression Pattern of Leptin and Its Receptors in Endometrioid Endometrial Cancer.
Expression Pattern of Leptin and Its Receptors in Endometrioid Endometrial Cancer.
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瘦素及其受体在子宫内膜样癌中的表达
DOI：
10.3390/jcm10132787
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发表时间：
2021-06-24
影响因子：
3.9
通讯作者：
Opławski M
中科院分区：
文献类型：
--
作者：
Boroń D;Nowakowski R;Grabarek BO;Zmarzły N;Opławski M
由于癌症发病率仍然很高，因此鉴定新的分子标记物和开发癌症治疗策略非常重要。肥胖可导致癌症进展，包括子宫内膜癌。脂肪细胞分泌瘦素，当瘦素处于高水平时，与癌症风险增加有关。本研究的目的是确定瘦素相关基因在子宫内膜组织样本和患者的全血中的表达谱。研究材料包括从30名子宫内膜癌患者和30名非癌症患者收集的组织样本和全血。微阵列用于评估瘦素相关基因的表达谱。然后，通过实时定量逆转录反应（RT-qPCR）测定瘦素（LEP）、瘦素受体（LEPR）、瘦素受体重叠转录物（LEPROT）和瘦素受体重叠转录物样1（LEPROTL 1）的表达。采用酶联免疫吸附试验（ELISA）检测血清瘦素浓度。瘦素及其受体在mRNA和蛋白质水平均过表达。此外，瘦素水平与患者体重指数（BMI）之间存在强正相关。瘦素及其受体水平升高可能有助于子宫内膜癌的进展。这些观察结果可能有助于设计子宫内膜癌治疗策略。
The identification of novel molecular markers and the development of cancer treatment strategies are very important as cancer incidence is still very high. Obesity can contribute to cancer progression, including endometrial cancer. Adipocytes secrete leptin, which, when at a high level, is associated with an increased risk of cancer. The aim of this study was to determine the expression profile of leptin-related genes in the endometrial tissue samples and whole blood of patients. The study material included tissue samples and whole blood collected from 30 patients with endometrial cancer and 30 without cancer. Microarrays were used to assess the expression profile of leptin-related genes. Then, the expression of leptin (LEP), leptin receptor (LEPR), leptin receptor overlapping transcript (LEPROT), and leptin receptor overlapping transcript-like 1 (LEPROTL1) was determined by the Real-Time Quantitative Reverse Transcription Reaction (RT-qPCR). The serum leptin concentration was evaluated using Enzyme-linked immunosorbent assay (ELISA). Leptin and its receptors were overexpressed both at the mRNA and protein levels. Furthermore, there were strong positive correlations between leptin levels and patient Body Mass Index (BMI). Elevated levels of leptin and its receptors may potentially contribute to the progression of endometrial cancer. These observations may be useful in designing endometrial cancer treatment strategies.
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影响因子：
6.4
作者：
Carino, Cecilia;Olawaiye, Alexander B.;Cherfils, Salandre;Serikawa, Takehiro;Lynch, Maureen P.;Rueda, Bo R.;Gonzalez, Ruben R.
通讯作者：
Gonzalez, Ruben R.
影响因子：
4.6
作者：
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通讯作者：
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影响因子：
3.9
作者：
Casadio, Paolo;Guasina, Francesca;Seracchioli, Renato
通讯作者：
Seracchioli, Renato
影响因子：
8.8
作者：
Helpman, L.;Kupets, R.;Nofech-Mozes, S.
通讯作者：
Nofech-Mozes, S.
影响因子：
3.3
作者：
Chen, Wenchao;Wu, Gang;Sun, Peichun
通讯作者：
Sun, Peichun