Gene Expression Profiling of Transcription Factors of Helicobacter pylori under Different Environmental Conditions
Gene Expression Profiling of Transcription Factors of Helicobacter pylori under Different Environmental Conditions
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DOI：
10.3389/fmicb.2017.00615
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发表时间：
2017-04-10
影响因子：
5.2
通讯作者：
Torres, Javier
中科院分区：
文献类型：
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作者：
De la Cruz, Miguel A.;Ares, Miguel A.;Torres, Javier
幽门螺杆菌是一种革兰氏阴性细菌，定植于人类胃粘膜并引起消化性溃疡和胃癌。H. pylori菌株26695具有小的基因组（1.67 Mb），其编码很少的已知的控制细菌代谢和毒力的转录调节因子。我们通过qRT-PCR分析了16个转录调控因子在H. pylori 26695，包括不同环境条件下的三个sigma因子。当细菌暴露于酸性pH、尿素、镍或铁时，σ因子差异表达，其中fliA的诱导特别强。调节基因hrcA，hup和crdR在尿素，镍和铁的存在下高度诱导。在生物膜形成方面，在固着细菌中诱导fliA、flgR、hp 1021、fur、nikR和crdR。rpoD、flgR、hspR、hp 1043和cheY的转录表达水平在与AGS上皮细胞接触时增加。卡那霉素、氯霉素和四环素增加或减少调节基因的表达，表明这些抗生素影响H。幽门。我们的数据表明，可能存在于人类胃中的环境线索调节H。pylori转录
Helicobacter pylori is a Gram-negative bacterium that colonizes the human gastric mucosa and causes peptic ulcers and gastric carcinoma. H. pylori strain 26695 has a small genome (1.67 Mb), which codes for few known transcriptional regulators that control bacterial metabolism and virulence. We analyzed by qRT-PCR the expression of 16 transcriptional regulators in H. pylori 26695, including the three sigma factors under different environmental conditions. When bacteria were exposed to acidic pH, urea, nickel, or iron, the sigma factors were differentially expressed with a particularly strong induction of fliA. The regulatory genes hrcA, hup, and crdR were highly induced in the presence of urea, nickel, and iron. In terms of biofilm formation fliA, flgR, hp1021, fur, nikR, and crdR were induced in sessile bacteria. Transcriptional expression levels of rpoD, flgR, hspR, hp1043, and cheY were increased in contact with AGS epithelial cells. Kanamycin, chloramphenicol, and tetracycline increased or decreased expression of regulatory genes, showing that these antibiotics affect the transcription of H. pylori. Our data indicate that environmental cues which may be present in the human stomach modulate H. pylori transcription.