A broad expression profile of the GMR-GAL4 driver in Drosophila melanogaster
A broad expression profile of the GMR-GAL4 driver in Drosophila melanogaster
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GMR-GAL4 驱动程序在果蝇中的广泛表达谱
DOI：
10.4238/2012.august.6.4
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发表时间：
2012-01-01
影响因子：
0.4
通讯作者：
Xue, L.
中科院分区：
文献类型：
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作者：
Li, W. -Z.;Li, S. -L.;Xue, L.
GAL4/UAS二元系统已广泛应用于果蝇中，以组织特异性方式异位表达转基因。 GMR-GAL4 驱动程序在玻璃多重报告基因 (GMR) 启动子元件的控制下表达酵母转录因子 GAL4，通常用于表达目标转基因，特别是在发育中的眼睛中。然而，我们观察到异常的翅膀表型；这是关键的翅膀发育基因（例如）活动的结果。例如，Notch 或 Wg 通路的成分，在 GMR-GAL4 驱动程序的控制下上调或下调。 X-gal 染色证实，当由 GMR-GAL4 驱动程序驱动时，UAS-LacZ 在三龄幼虫翼成虫盘以及眼盘中表达。此外，我们发现 GMR-GAL4 还驱动 UAS-LacZ 在其他组织中表达，例如大脑、气管和腿椎间盘。这些结果表明 GMR-GAL4 具有广泛的表达谱，而不是之前描述的眼睛特异性模式，并且在将其用作靶向基因表达的工具时应该小心。
The GAL4/UAS binary system has been widely used in Drosophila melanogaster for ectopic expression of transgenes in a tissue-specific manner. The GMR-GAL4 driver, which expresses the yeast transcription factor GAL4 under the control of glass multiple reporter (GMR) promoter elements, has been commonly utilized to express target transgenes, specifically in the developing eye. However, we have observed abnormal wing phenotypes; this is a result of the activity of critical wing developing genes, e. g., components of the Notch or Wg pathway, that are up-or down-regulated under the control of the GMR-GAL4 driver. X-gal staining confirmed that UAS-LacZ is expressed in third-instar larva wing imaginal discs, as well as in eye discs, when driven by the GMR-GAL4 driver. Furthermore, we found that GMR-GAL4 also drives UAS-LacZ expression in other tissues, such as brain, trachea, and leg discs. These results indicate that GMR-GAL4 has a broad expression profile, rather than the eye-specific pattern described previously, and that one should be careful when using it as a tool for targeted gene expression.