Aligned electrospun nanofibers specify the direction of dorsal root ganglia neurite growth
Aligned electrospun nanofibers specify the direction of dorsal root ganglia neurite growth
复制标题
DOI：
10.1002/jbm.a.31285
复制
发表时间：
2007-12-01
影响因子：
4.9
通讯作者：
Martin, David C.
中科院分区：
文献类型：
--
作者：
Corey, Joseph M.;Lin, David Y.;Martin, David C.
神经损伤，残疾的一个重要原因，可以更有效地治疗使用含有纵向排列的纤维神经引导通道。排列的电纺纳米纤维指导永生化神经干细胞的神经突生长，显示出指导再生神经突的潜力。然而，还没有研究这些纤维上的神经突引导与原代神经元。在这里，我们检查了高、中、随机排列的静电纺聚L-乳酸纳米纤维上来自背根神经节外植体的神经突。在对齐的纤维上，神经突从神经节径向向外生长，并在接触时转向跟随纤维。神经突的引导是稳健的，神经突从未离开纤维在周围的盖玻片上生长。为了比较神经突的对齐与神经纤维基质的对齐，使用傅立叶方法来量化对齐。神经突排列，但惊人的，是劣于纤维排列的所有，但随机排列的纤维。高度对齐的基板上的神经突分别比随机和中间纤维上的神经突长20%和16%。纤维上的雪旺细胞与周围盖玻片上的雪旺细胞相比呈非常狭窄的形态。这里展示的强大的神经突引导是使用对齐的电纺纳米纤维进行神经再生的重要一步。(C)2007 Wiley Periodicals，Inc.
Nerve injury, a significant cause of disability, may be treated more effectively using nerve guidance channels containing longitudinally aligned fibers. Aligned, electrospun nanofibers direct the neurite growth of immortalized neural stem cells, demonstrating potential for directing regenerating neurites. However, no study of neurite guidance on these fibers has yet been performed with primary neurons. Here, we examined neurites from dorsal root ganglia explants on electrospun poly-L-lactate nanofibers of high, intermediate, and random alignment. On aligned fibers, neurites grew radially outward from the ganglia and turned to follow the fibers upon contact. Neurite guidance was robust, with neurites never leaving the fibers to grow on the surrounding cover slip. To compare the alignment of neurites to that of the nanofiber substrates, Fourier methods were used to quantify the alignment. Neurite alignment, however striking, was inferior to fiber alignment on all but the randomly aligned fibers. Neurites on highly aligned substrates were 20 and 16% longer than neurites on random and intermediate fibers, respectively. Schwann cells on fibers assumed a very narrow morphology compared to those on the surrounding coverslip. The robust neurite guidance demonstrated here is a significant step toward the use of aligned, electrospun nanofibers for nerve regeneration. (C) 2007 Wiley Periodicals, Inc.