Spatio-temporal pattern for expression of galectin-3 in the murine utero-placental complex: evidence for differential regulation.
Spatio-temporal pattern for expression of galectin-3 in the murine utero-placental complex: evidence for differential regulation.
复制标题
小鼠子宫胎盘复合体中半乳糖凝集素 3 表达的时空模式：差异调节的证据。
DOI：
10.1095/biolreprod58.5.1277
复制
发表时间：
1998
影响因子：
3.6
通讯作者：
Weitlauf,HM
中科院分区：
文献类型：
--
作者：
Lee,VH;Lee,AB;Phillips,EB;Roberts,JK;Weitlauf,HM
在小鼠中，免疫反应性半乳糖凝集素-3蛋白先前已定位于邻近植入胚泡的子宫上皮细胞以及植入部位的蜕膜化子宫内膜、子宫自然杀伤细胞和几种类型的胎盘滋养层细胞中。由于半乳糖凝集素-3是一种可溶性细胞外分子，通过免疫组织化学方法的蛋白定位不能证明其细胞来源。因此，本研究进行，以确定子宫胎盘复合体中表达半乳糖凝集素-3mRNA的细胞类型。原位杂交结果表明，galectin-3 mRNA表达在整个子宫胎盘复合体中的所有细胞类型，以前显示含有免疫反应蛋白，包括子宫上皮细胞，蜕膜化子宫内膜，子宫自然杀伤细胞，胎盘滋养层细胞。这些结果表明，半乳糖凝集素-3蛋白不是在有限的细胞类型中合成的，而是通过细胞外间隙易位到其他组织隔室。此外，北方印迹分析的总RNA制备的子宫胎盘复合体的胎儿和母体成分分离证明了不同的模式的半乳糖凝集素-3 mRNA的表达在子宫和胎盘。半乳糖凝集素-3mRNA的相对水平在妊娠中期在着床部位达到峰值，在妊娠后半期在胎盘中保持升高，但在子宫中下降。指示了用于调节半乳糖凝集素-3在胎儿-母体界面的相对侧上的表达的单独机制。
In mice, immunoreactive galectin-3 protein has previously been localized in uterine epithelial cells adjacent to implanting blastocysts as well as in the decidualized endometrium of implantation sites, uterine natural killer cells, and several types of placental trophoblast cells. Because galectin-3 is a soluble extracellular molecule, protein localization by immunohistochemical methods does not demonstrate its cellular origin. Therefore, the present study was undertaken to determine precisely which cell types in the utero-placental complex express galectin-3mRNA. In situ hybridization results demonstrated that galectin- 3mRNA was expressed throughout the utero-placental complex in all cell types previously shown to contain immunoreactive protein, including uterine epithelium, decidualized endometrium, uterine natural killer cells, and placental trophoblasts. These results indicate that galectin-3 protein is not synthesized in a restricted cell type and translocated through the extracellular spaces to other tissue compartments. Furthermore, Northern blot analysis of total RNA prepared from separated fetal and maternal components of utero-placental complexes demonstrated different patterns of expression for galectin-3mRNA in the uterus and placenta. Relative levels of galectin-3mRNA peak at midgestation in the implantation site and during the second half of gestation remain elevated in the placenta but decline in the uterus. Separate mechanisms for regulating expression of galectin- 3 on opposite sides of the feto-maternal interface are indicated.