THE SEQUENCE OF THE DNAS CODING FOR THE MATING-TYPE LOCI OF SACCHAROMYCES-CEREVISIAE
THE SEQUENCE OF THE DNAS CODING FOR THE MATING-TYPE LOCI OF SACCHAROMYCES-CEREVISIAE
复制标题
DOI：
10.1016/0092-8674(81)90356-1
复制
发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
HALL, BD
中科院分区：
文献类型：
--
作者：
ASTELL, CR;AHLSTROMJONASSON, L;HALL, BD
酵母a交配型位点MATa以及沉默的α盒HMLα的完整序列已被确定。642个核苷酸的片段是MATa所特有的，747个核苷酸的相应片段是MATα所特有的。由MATa和MATα编码的主要mRNA（a1、a2、α1和α2）与DNA序列进行了比对。a1 mRNA完全在a特异性DNA序列内编码。与a1 mRNA反向转录的a2 mRNA在MATa和MATα共有的区域编码。α1和α2 mRNA也是反向转录的，并且它们的5′起始位点在α特异性序列内相距约240个核苷酸。根据DNA序列预测了MAT蛋白的氨基酸序列。一个出乎意料的结论是，含有148个氨基酸的a1蛋白是由密码子45处的UGA通读产生的。HMLα、MATα、MATa和HMRa序列同源外部片段的多态形式表明，参与交配型转换的片段边界紧邻a特异性和α特异性序列。
The complete sequences of the yeast a mating-type locus, MATa, and of the silent .alpha. cassette, HML.alpha., have been determined. A segment of 642 nucleotides is unique to MATa, and a corresponding segment of 747 nucleotides is unique to MAT.alpha.. The major mRNA (a1, a2, .alpha.1 and .alpha.2) encoded by MATa and MAT.alpha. were aligned with the DNA sequence. The a1 mRNA is encoded entirely within the a-specific DNA sequence. The a2 mRNA, which is transcribed divergently from a1 mRNA, is encoded in a region common to both MaTa and Mat.alpha.. The .alpha.1 and .alpha.2 mRNA are also transcribed divergently and have their 5'' starts about 240 nucleotides apart within the .alpha.-specific sequence. The amino acid sequences of the MAT proteins were predicted from the DNA sequences. An unanticipated conclusion is that the a1 protein, containing 148 amino acids, results from readthrough of a UGA at codon 45. Polymorphic forms of the homologous outer segments of the HML.alpha., MAT.alpha., MATa and HMRa sequences suggest that the boundaries of the segments involved in mating-typing switching are immediately adjacent to the a-specific and .alpha.-specific sequences.