Substance P receptor (neurokinin-1)-expressing neurons in lamina I of the spinal cord encode for the intensity of noxious stimulation: A c-Fos study in rat
Substance P receptor (neurokinin-1)-expressing neurons in lamina I of the spinal cord encode for the intensity of noxious stimulation: A c-Fos study in rat
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DOI：
10.1016/s0306-4522(98)00276-0
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发表时间：
1999-03-01
期刊：
影响因子：
3.3
通讯作者：
Hunt, SP
中科院分区：
文献类型：
--
作者：
Doyle, CA;Hunt, SP
P物质受体神经激肽-1由大鼠脊髓中的神经元亚群表达。我们结合免疫组化Fos，标记有害的外周刺激，和神经激肽-1，以检查是否伤害性信号从特定的外周组织（皮肤，肌肉或膝关节）或活动所产生的神经损伤或福尔马林诱导的炎症优先调制P物质。我们的研究结果表明，浅层和深层脊髓神经激肽-1-阳性神经元处理伤害性信息在显着不同的方式。在第一层，双标记的神经元的数量与刺激的强度呈正相关（由总Fos计数定义），并没有直接关系到任何特定的外周目标。然而，在脊髓的更深层（V-X）中，没有这种相关性，并且关节伤害感受器的刺激和福尔马林诱导的炎症产生了最大比例的Fos/神经激肽-1共定位，这表明P物质在介导关节疼痛和炎性痛觉过敏中的特殊作用。带有层I神经激肽-L受体的神经元似乎参与疼痛的强度辨别方面，而深部神经激肽-L细胞参与空间定位或特定伤害感受亚形式的检测。(C)1998年IBRO。出版社：Elsevier Science Ltd
The substance P receptor neurokinin-l is expressed by a subset of neurons in the rat spinal cord. We have combined immunostaining for Fos, a marker of noxious peripheral stimulation, and neurokinin-l to examine whether nociceptive signals From particular peripheral tissues (skin, muscle or knee joint) or activity generated by nerve injury or formalin-induced inflammation are preferentially modulated by substance P.Our results indicate that superficial and deep spinal neurokinin-1-positive neurons process nociceptive information in markedly different ways. In lamina I, the number of double-labelled neurons was positively correlated with the intensity of the stimulus (defined by the total Fos count) and was not directly related to any particular peripheral target. However, in the deeper layers of the spinal cord (V-X), there was no such correlation, and stimulation of joint nociceptors and formalin-induced inflammation produced the greatest proportion of Fos/neurokinin-1 co-localization, suggesting a particular role for substance P in the mediation of joint pain and inflammatory hyperalgesia.Thus, lamina I neurokinin-l receptor-bearing neurons appear to be involved in intensity discriminative aspects of pain, whereas the deep neurokinin-l cells are involved in spatial localization or the detection of particular nociceptive submodalities. (C) 1998 IBRO. Published by Elsevier Science Ltd.