Clinical and Pathological Implications of Increases in Tonsillar CD19+CD5+ B Cells, CD208+ Dendritic Cells, and IgA1-positive Cells of Immunoglobulin A Nephropathy
Clinical and Pathological Implications of Increases in Tonsillar CD19+CD5+ B Cells, CD208+ Dendritic Cells, and IgA1-positive Cells of Immunoglobulin A Nephropathy
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DOI：
10.1007/s11596-022-2532-5
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发表时间：
2022-02
影响因子：
2.4
通讯作者：
Yang Cai;Meiling Chen;Yuan-jun Deng;Le-le Liu;Xue-ping Lin;Ping-fan Lu;Yiyan Guo;Min Han
中科院分区：
文献类型：
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作者：
Yang Cai;Meiling Chen;Yuan-jun Deng;Le-le Liu;Xue-ping Lin;Ping-fan Lu;Yiyan Guo;Min Han
目的多项研究表明，扁桃体切除术可改善免疫球蛋白A肾病(IgAN)患者的预后。但扁桃体免疫与IgAN的关系尚不清楚。方法选择2015年5月至2016年4月在同济医院就诊的14例IgAN患者。用免疫荧光和免疫组织化学方法检测扁桃体中的B细胞、树突状细胞(DC)和IgA1阳性细胞。结果CD19+CD5+B细胞主要分布在淋巴滤泡的生发中心和套层，CD208+DC主要分布在滤泡间和上皮下区，IgA1阳性细胞主要分布在淋巴滤泡和上皮下组织的套层。IgAN患者扁桃体组织中CD19+CD5+B细胞、CD208+DC和IgA1+细胞数均显著高于正常对照组(P<0.01)。扁桃体组织中CD19+CD5+B细胞、CD208+DC和IgA1阳性细胞与24 h蛋白尿和肾小管萎缩/间质纤维化显著相关。结论扁桃体组织中CD19+CD5+B细胞、CD208+DC和IgA1阳性细胞可能参与了IgAN的发病过程。
ObjectiveSeveral studies indicated that tonsillectomy can improve the prognosis of patients with immunoglobulin A nephropathy (IgAN). However, the relationship between tonsillar immunity and IgAN is still unclear.MethodsA total of 14 IgAN patients were recruited in the current study from May 2015 to April 2016 in Tongji Hospital. B cells, dendritic cells (DCs), and IgA1 positive cells in human tonsils were detected using immunofluorescence and immunohistochemistry. Correlations between these cells and clinicopathologic features were evaluated.ResultsCD19+CD5+B cells were predominantly located in germinal centers and mantle zones of lymphoid follicles, the CD208+ DCs were distributed in the interfollicular and subepithelial area, and IgA1-positive cells were predominantly detected in mantle zones of lymphoid follicles and subepithelial tissues. The numbers of CD19+CD5+B cells, CD208+DCs, and IgA1-positive cells in tonsillar tissues from IgAN patients were significantly higher than those in the normal controls (P<0.01, respectively). CD19+CD5+B cells, CD208+DCs, and IgA1-positive cells in tonsillar tissues were significantly associated with 24-h proteinuria levels and tubular atrophy/interstitial fibrosis of IgAN.ConclusionCD19+CD5+B cells, CD208+DCs, and IgA1-positive cells in tonsillar tissues might be involved in the pathogenesis of IgAN.