Amino-acid levels in subretinal and vitreous fluid of patients with retinal detachment
Amino-acid levels in subretinal and vitreous fluid of patients with retinal detachment
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DOI：
10.1038/sj.eye.6702993
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发表时间：
2008-04-01
期刊：
影响因子：
3.9
通讯作者：
Arroyo, J. G.
中科院分区：
文献类型：
--
作者：
Bertram, K. M.;Bula, D. V.;Arroyo, J. G.
目的比较原发性孔源性视网膜脱离患者视网膜下液和玻璃体液中氨基酸的浓度，并与对照组玻璃体进行比较。方法本前瞻性观察性研究测定了20例接受巩膜扣带术的患者视网膜下液和5例接受睫状体平坦部玻璃体切除术的患者玻璃体液中氨基酸的浓度。来自接受黄斑裂孔（n = 7）或视网膜前膜（n = 3）的玻璃体切除术的患者的玻璃体液作为对照。使用高压液相色谱法分析视网膜下液和对照玻璃体。视网膜脱离玻璃体采用毛细血管荧光激光诱导荧光分析。（27.0+/-1.7 μ M），天冬氨酸（4.1+/-4.0 μ M），和甘氨酸与对照玻璃体相比，视网膜脱离中视网膜下液中谷氨酸（44.1+/-31.0 μ M）和玻璃体中谷氨酸（13.4+/-11.9 μ M）显著升高。视网膜下液中天冬氨酸和谷氨酸水平之间存在显著的正相关（斯皮尔曼相关系数：0.74，P
Purpose To compare the concentration of amino acids in subretinal and vitreous fluid of patients with primary rhegmatogenous retinal detachment to that of control vitreous.Methods This prospective, observational study measured amino-acid levels in subretinal fluid of patients undergoing scleral buckle placement (n = 20) and vitreous fluid in patients undergoing pars plana vitrectomy (n = 5) for primary retinal detachment. Vitreous fluid from patients undergoing vitrectomy for macular hole (n = 7) or epiretinal membrane (n = 3) served as a control. Subretinal fluid and control vitreous were analysed using high-pressure liquid chromatography. Retinal detachment vitreous was analysed using capillary electrophoresis-laser-induced fluorescence.Results Mean levels of glutamate (27.0+/-1.7 mu M), aspartate (4.1+/-4.0 mu M), and glycine (44.1+/-31.0 mu M) in subretinal fluid and glutamate (13.4+/-11.9 mu M) in the vitreous were significantly elevated in retinal detachment compared to control vitreous. A significant, positive association was observed between levels of aspartate and glutamate in subretinal fluid (Spearman's correlation coefficient: 0.74, P