THE HUMAN Y-CHROMOSOME - OVERLAPPING DNA CLONES SPANNING THE EUCHROMATIC REGION
THE HUMAN Y-CHROMOSOME - OVERLAPPING DNA CLONES SPANNING THE EUCHROMATIC REGION
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DOI：
10.1126/science.1359640
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发表时间：
1992-10-02
期刊：
影响因子：
56.9
通讯作者：
PAGE, DC
中科院分区：
文献类型：
--
作者：
FOOTE, S;VOLLRATH, D;PAGE, DC
通过将196个重组DNA克隆（每个克隆包含染色体的一个片段）组装成单个重叠阵列，对人类Y染色体进行物理作图。该阵列包括Y染色体的常染色质部分的98%以上。首先，从人XYYYY男性的基因组DNA制备酵母人工染色体（YAC）克隆文库。筛选文库以鉴定含有160个序列标记位点的克隆，然后根据这些信息构建图谱。 总之，207个Y染色体DNA基因座被分配到127个有序的间隔的基础上，它们的存在或不存在的YAC的，产生有序的地标在平均间距为220个碱基在整个常染色质区域。该图谱揭示了Y染色体基因分散在X同源、Y特异性重复和单拷贝DNA序列的拼凑物中。这张重叠克隆和有序、密集分布的标记的图谱应该会加速染色体的研究。
The human Y chromosome was physically mapped by assembling 196 recombinant DNA clones, each containing a segment of the chromosome, into a single overlapping array. This array included more than 98 percent of the euchromatic portion of the Y chromosome. First, a library of yeast artificial chromosome (YAC) clones was prepared from the genomic DNA of a human XYYYY male. The library was screened to identify clones containing 160 sequence-tagged sites and the map was then constructed from this information. In all, 207 Y-chromosomal DNA loci were assigned to 127 ordered intervals on the basis of their presence or absence in the YAC's, yielding ordered landmarks at an average spacing of 220 kilobases across the euchromatic region. The map reveals that Y-chromosomal genes are scattered among a patchwork of X-homologous, Y-specific repetitive, and single-copy DNA sequences. This map of overlapping clones and ordered, densely spaced markers should accelerate studies of the chromosome.