Prevalence of extended-spectrum β-lactamase-producing Gram-negative bacteria in septicaemic neonates in a tertiary care hospital
Prevalence of extended-spectrum β-lactamase-producing Gram-negative bacteria in septicaemic neonates in a tertiary care hospital
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DOI：
10.1099/jmm.0.04966-0
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发表时间：
2003-05-01
影响因子：
3
通讯作者：
Agarwal, SK
中科院分区：
文献类型：
--
作者：
Jain, A;Roy, I;Agarwal, SK
本研究旨在探讨多重耐药革兰阴性杆菌引起新生儿败血症的高发病率。对728例疑似病例的新生儿血进行了多茴香脑磺酸钠脑心浸液培养。根据NCCLS的建议，随后对分离的所有革兰氏阴性杆菌进行常规抗菌药物敏感性试验和超广谱β-内酰胺酶（ESBL）生产试验。克雷伯菌属ESBL检出率为86.6%，肠杆菌属占73.4%。大肠埃希菌占63.6%。在常规药敏试验中，74.4%~ 80.9%的产ESBL菌对头孢噻肟耐药，47.6%~ 59.5%的产ESBL菌对头孢他啶耐药。发现一些产ESBL菌（36.3-61.5%）对本研究中使用的一种或两种头孢菌素敏感。结论：新生儿重症监护病房（NICU）中ESBL多重耐药菌株的选择可能与第三代头孢菌素的滥用有关。
The present study was undertaken to investigate the high incidence of multiresistant Gram-negative bacilli causing neonatal septicaemia. Samples of neonatal blood from 728 suspected cases were obtained in brain heart infusion broth with sodium polyanethol sulfonate. All Gram-negative rods isolated were subsequently subjected to routine antimicrobial susceptibility testing and tests for extended-spectrum P-lactamase (ESBL) production, as per NCCLS recommendations. ESBL was detected in 86.6% of Klebsiella spp., 73.4% of Enterobacter spp. and 63.6% of Escherichia coli strains. It was also observed that 74.4-80.9% of these ESBL producers were resistant to cefotaxime and 47.6-59.5% were resistant to ceftazidime in routine susceptibility testing. Some ESBL producers (36.3-61.5%) were found to be susceptible to either or both cephalosporins used in this study. It is concluded that indiscriminate use of third-generation cephalosporins may be responsible for the selection of ESBL-producing multiresistant strains in the neonatal intensive-care unit (NICU).