Lift-off cell lithography for cell patterning with clean background

Lift-off cell lithography for cell patterning with clean background
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DOI:
10.1039/c8lc00726h
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发表时间:
2018-10-21
期刊:
影响因子:
6.1
通讯作者:
Chiou, Pei-Yu
Chiou, Pei-Yu
中科院分区:
工程技术1区
文献类型:
--
作者:
Wu, Cong;Zhu, Xiongfeng;Chiou, Pei-Yu

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我们开发了一种高效的方法图案化细胞的一种新颖而简单的技术称为剥离细胞光刻(LCL)。我们的方法借用了微制造的剥离光刻的关键概念,并利用完全生物相容的工艺来实现高通量,高效率的细胞图案化,在大的表面积上具有几乎为零的背景缺陷。使用LCL,我们可重复地实现了粘附和非粘附细胞的>70%的图案化效率,
We developed a highly efficient method for patterning cells by a novel and simple technique called lift-off cell lithography (LCL). Our approach borrows the key concept of lift-off lithography from microfabrication and utilizes a fully biocompatible process to achieve high-throughput, high-efficiency cell patterning with nearly zero background defects across a large surface area. Using LCL, we reproducibly achieved >70% patterning efficiency for both adherent and non-adherent cells with