An ice-templated, linearly aligned chitosan-alginate scaffold for neural tissue engineering
An ice-templated, linearly aligned chitosan-alginate scaffold for neural tissue engineering
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DOI：
10.1002/jbm.a.34668
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发表时间：
2013-12-01
影响因子：
4.9
通讯作者：
Wheatley, Margaret A.
中科院分区：
文献类型：
--
作者：
Francis, Nicola L.;Hunger, Philipp M.;Wheatley, Margaret A.
已经研究了几种策略来增强脊髓损伤后的轴突再生，然而，所产生的生长可能是随机和无序的。生物工程支架为引导再生轴突朝向其靶点提供了物理基质，并且可以通过冷冻铸造产生。该技术涉及水溶液或悬浮液的受控定向固化，导致由冰模板引起的线性排列的多孔结构。本研究利用冷冻铸造法制备具有纵向孔道的壳聚糖-海藻酸钠（C/A）多孔支架。鸡背根神经节外植体粘附在支架上，并与通道方向平行对齐地延伸穿过支架的神经突。聚阳离子和层粘连蛋白的表面吸附促进了比未涂覆支架显著更长的神经突生长（聚-L-鸟氨酸+层粘连蛋白= 793.2 +/- 187.2 μ m;聚-L-赖氨酸+层粘连蛋白= 768.7 +/- 241.2 μ m;未涂覆支架= 22.52 +/- 50.14 μ m）（P < 0.001）。水合支架的弹性模量测定为5.08 +/- 0.61 kPa，与报告的脊髓值相当。目前的数据表明，这种C/A支架是一个有前途的候选人用作神经引导支架，因为它的能力，以支持神经元的附着和DRG神经突起的线性排列的生长。(c)2013 Wiley Periodicals，Inc. J Biomed Mater Res Part A：101A：3493-3503，2013.
Several strategies have been investigated to enhance axonal regeneration after spinal cord injury, however, the resulting growth can be random and disorganized. Bioengineered scaffolds provide a physical substrate for guidance of regenerating axons towards their targets, and can be produced by freeze casting. This technique involves the controlled directional solidification of an aqueous solution or suspension, resulting in a linearly aligned porous structure caused by ice templating. In this study, freeze casting was used to fabricate porous chitosan-alginate (C/A) scaffolds with longitudinally oriented channels. Chick dorsal root ganglia explants adhered to and extended neurites through the scaffold in parallel alignment with the channel direction. Surface adsorption of a polycation and laminin promoted significantly longer neurite growth than the uncoated scaffold (poly-L-ornithine + Laminin = 793.2 +/- 187.2 mu m; poly-L-lysine + Laminin = 768.7 +/- 241.2 mu m; uncoated scaffold = 22.52 +/- 50.14 mu m) (P < 0.001). The elastic modulus of the hydrated scaffold was determined to be 5.08 +/- 0.61 kPa, comparable to reported spinal cord values. The present data suggested that this C/A scaffold is a promising candidate for use as a nerve guidance scaffold, because of its ability to support neuronal attachment and the linearly aligned growth of DRG neurites. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3493-3503, 2013.