Molecular diversity of LysM carbohydrate-binding motifs in fungi.
Molecular diversity of LysM carbohydrate-binding motifs in fungi.
复制标题
DOI：
10.1007/s00294-014-0471-9
复制
发表时间：
2015-05
期刊：
影响因子：
2.5
通讯作者：
Seidl-Seiboth, Verena
中科院分区：
文献类型：
--
作者：
Akcapinar, Gunseli Bayram;Kappel, Lisa;Sezerman, Osman Ugur;Seidl-Seiboth, Verena
LysM基序是在原核生物和真核生物中发现的碳水化合物结合模块。它们与含N-乙酰葡糖胺的碳水化合物结合，如甲壳素、甲壳低聚糖和肽聚糖。本文综述了真菌中含LysM蛋白的结构特征，并讨论了它们的生化特性、转录谱和生物学功能。此外，基于真菌LysM基序的进化分析和共识模式分析的数据，我们表明，它们可以被分类为真菌特异性组和真菌/细菌组。这有利于进一步的LysM蛋白的分类和选择进行详细的分析，并将有助于扩大我们对真菌中该蛋白家族的功能谱的理解。真菌LysM基序主要存在于亚群C几丁质酶和LysM效应蛋白中，其是具有LysM基序但没有催化结构域的分泌蛋白。在酶中，LysM基序介导与不溶性碳源的附着。在植物中，含有LysM基序的受体负责感知甲壳素低聚糖，并参与植物与细菌或真菌之间有益的共生相互作用以及植物防御反应。在植物病原真菌中，LysM效应蛋白已被证明在抑制宿主防御反应以及真菌菌丝对几丁质酶的保护功能中具有重要功能。然而，大量的和多样性的蛋白质与LysM基序，正在解开真菌基因组测序项目表明，在真菌中的LysM效应蛋白的功能库，只有部分发现到目前为止。
LysM motifs are carbohydrate-binding modules found in prokaryotes and eukaryotes. They bind to N-acetylglucosamine-containing carbohydrates, such as chitin, chitio-oligosaccharides and peptidoglycan. In this review, we summarize the features of the protein architecture of LysM-containing proteins in fungi and discuss their so far known biochemical properties, transcriptional profiles and biological functions. Further, based on data from evolutionary analyses and consensus pattern profiling of fungal LysM motifs, we show that they can be classified into a fungal-specific group and a fungal/bacterial group. This facilitates the classification and selection of further LysM proteins for detailed analyses and will contribute to widening our understanding of the functional spectrum of this protein family in fungi. Fungal LysM motifs are predominantly found in subgroup C chitinases and in LysM effector proteins, which are secreted proteins with LysM motifs but no catalytic domains. In enzymes, LysM motifs mediate the attachment to insoluble carbon sources. In plants, receptors containing LysM motifs are responsible for the perception of chitin-oligosaccharides and are involved in beneficial symbiotic interactions between plants and bacteria or fungi, as well as plant defence responses. In plant pathogenic fungi, LysM effector proteins have already been shown to have important functions in the dampening of host defence responses as well as protective functions of fungal hyphae against chitinases. However, the large number and diversity of proteins with LysM motifs that are being unravelled in fungal genome sequencing projects suggest that the functional repertoire of LysM effector proteins in fungi is only partially discovered so far.
登录
查看更多内容
影响因子：
14.9
作者：
Grigoriev IV;Nikitin R;Haridas S;Kuo A;Ohm R;Otillar R;Riley R;Salamov A;Zhao X;Korzeniewski F;Smirnova T;Nordberg H;Dubchak I;Shabalov I
通讯作者：
Shabalov I
影响因子：
2.8
作者：
BIRKELAND, NK
通讯作者：
BIRKELAND, NK
发现具有任意插入和删除的序列基序。
影响因子：
4.3
作者：
Frith, Martin C.;Saunders, Neil F. W.;Kobe, Bostjan;Bailey, Timothy L.
通讯作者：
Bailey, Timothy L.
影响因子：
3.6
作者：
Bolton, Melvin D.;van Esse, H. Peter;Thomma, Bart P. H. J.
通讯作者：
Thomma, Bart P. H. J.
影响因子：
14.9
作者：
GARVEY, KJ;SAEDI, MS;ITO, J
通讯作者：
ITO, J