Hydrogen sulfide mediated the melatonin induced stoma closure by regulating the K+ channel in Arabidopsis thaliana
Hydrogen sulfide mediated the melatonin induced stoma closure by regulating the K+ channel in Arabidopsis thaliana
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DOI：
10.1016/j.envexpbot.2022.105125
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发表时间：
2022-11
影响因子：
5.7
通讯作者：
Zhiqing Wang;Y. Mu;Lu Zhang;Zhiqiang Liu;Danmei Liu;Zhuping Jin;Y. Pei
中科院分区：
文献类型：
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作者：
Zhiqing Wang;Y. Mu;Lu Zhang;Zhiqiang Liu;Danmei Liu;Zhuping Jin;Y. Pei
气体递质硫化氢（H2S）和植物激素褪黑激素（MEL）在增强植物抗旱性中的作用已被越来越多的人所了解，但其响应干旱的具体调控机制尚不清楚。本研究探讨了它们在干旱胁迫下气孔运动调节中的关系。在正常和干旱胁迫条件下，外源MEL均能提高H2S含量、产量以及H2S产生相关基因LCD和DES 1的转录水平。在lcd、des 1和lcd/des 1突变体中，MEL提高耐旱性的能力受到抑制，这表明MEL在此过程中部分依赖于H2S。外源MEL和H2S可提高干旱胁迫下与干旱相关的转录因子CBF 2、CBF 3、RD 29 A、DREB 2A和DREB 2Bin的转录水平，H2S和MEL可影响K+通道相关基因KCO 1、AKT 1、AKT 2、KAT 1和KC 1的转录水平，从而调节气孔运动。在翻译后水平，H2S增加了KCO 1和AKT 1的过硫化水平，降低了它们的降解速率。结果表明，内源H2S和MEL含量对kco 1、kat 1、akt 1和gork突变体均无影响，说明H2S通过调节K+通道介导MEL诱导气孔关闭，从而提高拟南芥的抗旱性。
The roles of the gasotransmitter hydrogen sulfide (H2S) and the phytohormone melatonin (MEL) in enhancing plant drought resistance are increasingly understood, but the specific regulatory mechanisms in response to drought remain unclear. This study investigated their relationship in the regulation of stomatal movement in response to drought stress. Under normal and drought stress conditions, exogenous MEL increased the H2S content, production rate and the transcription level of H2S production-related encoding genes LCD and DES1. The ability of MEL to increase drought stress tolerance was inhibited significantly inlcd, des1 and lcd/des1 mutants, which implied that MEL was partially dependent on H2S in this process. Exogenous MEL and H2S increased the transcriptional level of drought related transcription factorsCBF2,CBF3,RD29A,DREB2AandDREB2Bin response to drought stress and H2S and MEL affected the transcription level of K+channel-related genesKCO1,GORK,AKT1,AKT2,KAT1, andKC1to regulate stomatal movement. At the post-translational level, H2S increased the persulfidation levels of KCO1 and AKT1 and reduced their degradation rate. It was also found that endogenous H2S and MEL content had no effect on the mutants ofkco1,kat1,akt1andgork.It was concluded that H2S mediated the MEL induced stoma closure by regulating the K+channel to enhance plant drought resistance in Arabidopsis.