Analgesic effects of endomorphin-1 and endomorphin-2 in the formalin test in mice.
Analgesic effects of endomorphin-1 and endomorphin-2 in the formalin test in mice.
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DOI：
10.1016/s0024-3205(00)00689-5
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发表时间：
2000-07
期刊：
影响因子：
6.1
通讯作者：
R. Soignier;Anthony L. Vaccarino;Angela M. Brennan;A. Kastin;J. Zadina
中科院分区：
文献类型：
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作者：
R. Soignier;Anthony L. Vaccarino;Angela M. Brennan;A. Kastin;J. Zadina
在甩尾实验中，最近分离的两种肽，内啡肽-1 （Tyr-Pro-Trp-Phe-NH2）和内啡肽-2 （Tyr-Pro-Phe-Phe-NH2）是高选择性μ-阿片受体激动剂，具有镇痛作用。为了进一步评估这些肽的镇痛特性，在福尔马林试验中检测了内啡肽-1、内啡肽-2和吗啡的作用。用内啡肽-1、内啡肽-2或吗啡（0、1、3、10 μg）灌胃雄性瑞士韦氏小鼠，然后在一只后爪足底表面注射5%福尔马林s.c. 20 μl。注射福尔马林后观察60 min。内啡肽-1和内啡肽-2产生剂量依赖性镇痛持续时间比吗啡短。吗啡后运动能力增强，而内啡肽-1和内啡肽-2后运动能力增强。这些发现扩展了先前的结果，并提示内啡肽可能具有治疗急性疼痛的治疗潜力。
Two recently isolated peptides, endomorphin-1 (Tyr-Pro-Trp-Phe-NH2) and endomorphin-2 (Tyr-Pro-Phe-Phe-NH2), are highly selective μ-opioid receptor agonists with analgesic actions in the tail-flick test. To further assess the analgesic properties of these peptides, the effects of endomorphin-1, endomorphin-2, and morphine were examined in the formalin test. Male Swiss Webster mice were injected i.c.v. with endomorphin-1, endomorphin-2, or morphine (0, 1, 3, 10 μg) 5 min before injection of 20 μl of 5% formalin s.c. into the plantar surface of one hind-paw. The mice were observed for 60 min after formalin injection. Endomorphin-1 and endomorphin-2 produced dose-dependent analgesia that was shorter in duration than for morphine. Increased locomotion was observed after morphine, but not after endomorphin-1 or endomorphin-2. These findings extend previous results and suggest that endomorphins may have therapeutic potential for the treatment of acute pain.