Effects of confluence on phosphate transport capacity in cultured renal cell lines.
Effects of confluence on phosphate transport capacity in cultured renal cell lines.
复制标题
汇合对培养肾细胞系磷酸盐转运能力的影响。
DOI：
10.1002/jcp.1041350117
复制
发表时间：
1988
影响因子：
5.6
通讯作者：
Scheinman,SJ
中科院分区：
文献类型：
--
作者：
Scheinman,SJ
The LLC‐PK1cell line transports phosphate (Pi), glucose, and amino acids using carriers similar to those in proximal tubular cells. Others have reported that when monolayers reach confluence, hexose transport increases and activity of the A‐amino acid transporter falls. The present study evaluates Pi uptake by two continuous cell lines derived from renal proximal tubule, and demonstrates that phosphate uptake falls sharply upon reaching confluence in LLC‐PK1cells but not in cultured opossum kidney (OK) cells. The fall in Pi uptake in LLC‐PK1cells at confluence represents a halving in Vmaxfor Na‐dependent phosphate uptake (2.33 vs. 5.00 nmol/mg protein/5 min) without a change in Km (82 vs. 94 μM). Suppression of phosphate transport in confluent monolayers of LLC‐PK1cells is completely reversed by bringing the cells into suspension. As has been shown for the phorbol ester 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), exposure of monolayers to serum stimulates phosphate uptake, but unlike phorbol ester, serum does so without stimulating alanine uptake. OK cells differ from LLC‐PK1in that no change occurs in Pi uptake at confluence, although they resemble LLC‐PK1cells in that sugar uptake rises and alanine uptake falls at confluence. The different temporal patterns for Pi uptake in the two cell lines indicates that developmental change in the uptake of Pi is not linked to that of glucose or alanine.
