Resistance in melon to Cucurbit chlorotic yellows virus, a whitefly-transmitted crinivirus
Resistance in melon to Cucurbit chlorotic yellows virus, a whitefly-transmitted crinivirus
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DOI：
10.1007/s10658-012-0088-0
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发表时间：
2013-02-01
影响因子：
1.8
通讯作者：
Iwai, Hisashi
中科院分区：
文献类型：
--
作者：
Okuda, Shiori;Okuda, Mitsuru;Iwai, Hisashi
对原产于印度、巴基斯坦和孟加拉国的51份甜瓜（Cucumis melo）材料进行了瓜绿黄病毒（Cucurbit chlorotic yellow virus， CCYV）抗性评价。用q型甘薯粉虱（烟粉虱）接种CCYV后，接种株JP 138332、JP 216154、JP 216155、JP 216751和JP 91204均无症状或症状轻微，但通过逆转录聚合酶链反应（RT-PCR）在未接种的上部叶片中检测到CCYV。采用定量RT-PCR方法分析CCYV RNA的相对积累情况。除JP 138332外，所有品种的CCYV RNA积累水平与作为对照的商业品种Earl’s Seine相当。jp138332显示出较低的CCYV RNA积累。JP 138332上Q型烟粉虱的数量与Earl’s Seine上的数量无明显差异，表明该品种对CCYV的抗性不是由抗虫性引起的。因此，有5个品种对抗性品种的开发具有重要意义。特别是，JP 138332对CCYV具有很好的抗性，这可能与抑制病毒增殖有关。
Fifty-one melon (Cucumis melo) accessions that originated from India, Pakistan and Bangladesh were evaluated for resistance to Cucurbit chlorotic yellows virus (CCYV), a newly emerged species in the genus Crinivirus. CCYV was inoculated using sweet potato whitefly (Bemisia tabaci) biotype Q. Accessions, JP 138332, JP 216154, JP 216155, JP 216751 and JP 91204, showed no or faint symptoms, although CCYV was detected from the non-inoculated upper leaves by reverse transcription-polymerase chain reaction (RT-PCR). The five accessions were subjected to quantitative RT-PCR to analyze relative accumulation of CCYV RNA. All accessions except JP 138332 had levels of CCYV RNA accumulation comparable to the commercial variety, 'Earl's Seine', which was used as a control. JP 138332 showed a much lower CCYV RNA accumulation. Numbers of B. tabaci biotype Q on JP 138332 did not differ from 'Earl's Seine', in antixenosis tests, and the result suggested the resistance to CCYV was not due to antixenosis. Consequently, five accessions are of interest for development of resistant varieties. In particular, JP 138332 possesses a promising resistant trait for CCYV, which might be associated with inhibition of virus multiplication.