Age differences in the expression of metabotropic glutamate receptor 1 and inositol 1,4,5-trisphosphate receptor in mouse cerebellum.
Age differences in the expression of metabotropic glutamate receptor 1 and inositol 1,4,5-trisphosphate receptor in mouse cerebellum.
复制标题
小鼠小脑代谢型谷氨酸受体1和肌醇1,4,5-三磷酸受体表达的年龄差异。
DOI：
10.1016/s0304-3940(98)00127-x
复制
发表时间：
1998
影响因子：
2.5
通讯作者：
Sun,GY
中科院分区：
文献类型：
--
作者：
Simonyi,A;Xia,J;Igbavboa,U;Wood,WG;Sun,GY
用5月龄、15月龄和24月龄雄性C57BL/6NNIA小鼠研究了小脑代谢性谷氨酸受体1(MGluR1)和三磷酸肌醇受体(IP3R)表达的年龄差异。颗粒细胞层mGluR1mRNA的原位杂交显示，24月龄组的mGluR1mRNA水平显著高于5月龄组和15月龄组。然而，单个浦肯野神经元的信使核糖核酸水平没有年龄差异。使用针对主要亚型mGluR1a的抗体的Western印迹分析显示，24个月大的动物的蛋白质水平下降。原位杂交显示，24月龄组浦肯野神经元IP3R-1mRNA表达略有下降，但不明显。进一步测定[~3H]IP3与小脑膜结合的结果显示，与5月龄组相比，15和24月龄组的Bmax显著降低，但Kd值没有变化。MGluR1a受体蛋白的减少和IP3R结合位点的减少可能在小脑功能随增龄而下降的过程中起重要作用。
Age differences in the expression of cerebellar metabotropic glutamate receptor 1 (mGluR1) and inositol 1,4,5-trisphosphate receptor (IP3R) were investigated using male C57BL/6NNIA mice 5, 15 and 24 months of age. In situ hybridization for mGluR1 mRNA in the granule cell layer indicated significantly higher mRNA levels in the 24-month-old group as compared to the 5- and 15-month-old groups. However, mRNA levels of individual Purkinje neurons did not show age differences. Western blot analysis using antibody against the predominant isoform, mGluR1a, showed a decline in protein levels in the 24-month-old animals. In situ hybridization for IP3R type 1 mRNA in Purkinje neurons showed a slight but not significant decline in the 24-month-old group. Further assay of [3H]IP3binding with cerebellar membranes showed significant reduction in Bmaxvalues in the 15- and 24-month-old groups as compared to the 5-month-old group but Kdvalues were not changed. The decrease in mGluR1a receptor protein together with reduction in IP3R binding sites may play an important role in the decline in cerebellar functions with increasing age.