Liquiritinapioside - A mineralocorticoid-like substance from liquorice
Liquiritinapioside - A mineralocorticoid-like substance from liquorice
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DOI：
10.1016/j.foodchem.2019.03.056
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发表时间：
2019-08-15
期刊：
影响因子：
8.8
通讯作者：
Li, Yubo
中科院分区：
文献类型：
--
作者：
Fan, Simiao;Gu, Kun;Li, Yubo
对甘草中主要成分进行了表面等离子体共振（SPR）分析，并报道了一种新的强盐皮质激素受体（MR）激动剂甘草皂苷（LA），其结合常数为1.093 × 10 ~（-5）M。作为补充，通过竞争结合实验进一步证明LA具有较强的MR结合能力（LA的富集因子为10.22%）。本研究还验证了LA对H9 c2细胞的活性。用Ⅰ型胶原的表达和Masson染色的结果来确定该物质引起H9 c2细胞纤维化的能力。并采用蛋白质印迹法验证化合物诱导心肌纤维化的机制。总之，所获得的结果表明，LA可以通过MR诱导TGF-β 1/p38 MAPK信号通路的激活，从而诱导H9 c2细胞纤维化。
Surface plasmon resonance (SPR) analysis of the main components of liquorice was performed and a novel strong mineralocorticoid receptor (MR) agonist, namely liquiritinapioside (LA), whose the binding constant was 1.093 x 10(-5) M, was reported. As a supplement, LA has been further demonstrated to have a strong MR binding capacity through competitive binding experiments (the enrichment factor of LA was 10.22%). This study also validated the activity of LA on H9c2 cells. The expression of collagen I and the results of Masson staining were used to determine the ability of this substance to cause H9c2 cell fibrosis. Moreover, western blotting was used to verify the mechanism of compound-induced myocardial fibrosis. Overall, the attained results showed that LA could induce the activation of the TGF-beta 1/p38 MAPK signalling pathway through the MR to induce H9c2 cell fibrosis.