Isolation and Patch-Clamp of Primary Adipocytes.
Isolation and Patch-Clamp of Primary Adipocytes.
复制标题
原代脂肪细胞的分离和膜片钳。
DOI：
10.1007/978-1-4939-6820-6_14
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Sah,Rajan
中科院分区：
文献类型：
--
作者：
Zhang,Yanhui;Tong,Dan;Mishra,Anil;Xie,Litao;Samuel,Isaac;Smith,JessicaK;Sah,Rajan
The patch-clamp technique allows for the study of ion channel activity in the native adipocyte environment to better understand the contributions of ion channels to adipocyte signaling. Here, we describe methods for isolating primary mature adipocytes from both mouse and human white adipose tissues (subcutaneous and visceral). From the same preparation, we describe how to culture and differentiate preadipocytes isolated from the stromal vascular fraction. We then describe in detail patch-clamp methods, including both whole-cell and perforated-patch configurations.
