Biodegradation of 4-nitrophenol by indigenous microbial populations in Everglades soils
Biodegradation of 4-nitrophenol by indigenous microbial populations in Everglades soils
复制标题
DOI：
10.1023/a:1008285717595
复制
发表时间：
1997-01-01
期刊：
影响因子：
3.6
通讯作者：
Petrova, KP
中科院分区：
文献类型：
--
作者：
Laha, S;Petrova, KP
佛罗里达南部的大沼泽地是一个独特的生态系统。由于在这些湿地上游的农业地区广泛使用杀虫剂和除草剂，大沼泽地有严重的污染问题的可能性。本研究的目的是评估土著微生物种群降解农业和其他活动引入的外源性有机化合物的能力。这种生物降解可能有助于大沼泽地污染土壤和水的补救。本研究中选择的模型化合物是4-硝基苯酚，一种常用于生产农药的化学品。研究了不同浓度的4-硝基苯酚在大沼泽地土壤中的矿化作用。在10和100微克/克土壤的浓度下，一周内发生了相当大的矿化作用。在较高浓度下，即，10毫克/克土壤，然而，没有矿化的4-硝基苯酚发生超过4个月的时间;这样的高浓度显然产生了抑制作用。4-硝基苯酚矿化的速率和程度增强接种与先前分离的硝基苯酚降解微生物。然而，测得的最大矿化程度小于30%，表明转化为生物质和/或未鉴定的中间产物。这些结果表明，潜在的自然机制，以减轻在一个复杂的系统，如大沼泽地外源性污染物的不利影响。
The Everglades in South Florida are a unique ecological system. As a result of the widespread use of pesticides and herbicides in agricultural areas upstream from these wetlands, there is a serious potential for pollution problems in the Everglades. The purpose of this study was to evaluate the ability of indigenous microbial populations to degrade xenobiotic organic compounds introduced by agricultural and other activities. Such biodegradation may facilitate the remediation of contaminated soils and water in the Everglades. The model compound selected in this study is 4-nitrophenol, a chemical commonly used in the manufacture of pesticides. The mineralization of 4-nitrophenol at various concentrations was studied in soils collected from the Everglades. At concentrations of 10 and 100 mu g/g soil, considerable mineralization occurred within a week. At a higher concentration, i.e., 10 mg/g soil, however, no mineralization of 4-nitrophenol occurred over a 4-month period; such a high concentration apparently produced an inhibitory effect. The rate and extent of 4-nitrophenol mineralization was enhanced on inoculation with previously isolated nitrophenol-degrading microorganisms. The maximum mineralization extent measured, however, was less than 30% suggesting conversion to biomass and/or unidentified intermediate products. These results indicate the potential for natural mechanisms to mitigate the adverse effects of xenobiotic pollutants in a complex system such as the Everglades.