A Scalable “Junction Substrate” to Engineer Robust DNA Circuits
A Scalable “Junction Substrate” to Engineer Robust DNA Circuits
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用于设计稳健 DNA 电路的可扩展“结基板”
DOI：
10.1021/jacs.8b05203
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发表时间：
2018
影响因子：
15
通讯作者：
Haojun Liang
中科院分区：
文献类型：
--
作者：
Xianbao Sun;Bing Wei;Yijun Guo;Shiyan Xiao;Xiang Li;Dongbao Yao;Xue Yin;Shiyong Liu;Haojun Liang
多功能的构建模块对于构建复杂和规模化的DNA电路至关重要。在这项研究中，我们提出了一个概念上的新的可扩展的架构称为“结基板”（J-基板），是由预纯化的双链DNA分子连接。作为概念验证，这种新型基底已被用于构建多输入DNA电路，提供了优于传统基底（称为“线性基底”，L-基底）的几个优点。首先，J-底物不需要长的DNA链，从而避免了显著的合成错误和成本。其次，传统的PAGE纯化方法在技术上更容易获得高纯度的底物，从而有效地消除了初始泄漏。第三，通过引入“结”消除了渐近泄漏。最后，优化的J-基板架构的电路表现出快速的动力学。我们相信，所提出的架构构成了一个复杂的底盘构造复杂的电路。
Versatile building blocks are essential for building complex and scaled-up DNA circuits. In this study, we propose a conceptually new scalable architecture called a “junction substrate” (J-substrate) that is linked by prepurified double-stranded DNA molecules. As a proof-of-concept, this novel type of substrate has been utilized to build multi-input DNA circuits, offering several advantages over the conventional substrate (referred to as a “linear substrate”, L-substrate). First, the J-substrate does not require long DNA strands, thus avoiding significant synthetic errors and costs. Second, the traditional PAGE purification method is technically facilitated to obtain high-purity substrates, whereby the initial leakage is effectively eliminated. Third, the asymptotic leakage is eliminated by introducing the “junction”. Finally, circuits with the optimized J-substrate architecture exhibit fast kinetics. We believe that the proposed architecture constitutes a sophisticated chassis for constructing complex circuits.