POLARITY ORIENTATION OF MICROTUBULES IN HIPPOCAMPAL-NEURONS - UNIFORMITY IN THE AXON AND NONUNIFORMITY IN THE DENDRITE
POLARITY ORIENTATION OF MICROTUBULES IN HIPPOCAMPAL-NEURONS - UNIFORMITY IN THE AXON AND NONUNIFORMITY IN THE DENDRITE
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DOI：
10.1073/pnas.85.21.8335
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
BANKER, GA
中科院分区：
文献类型：
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作者：
BAAS, PW;DEITCH, JS;BANKER, GA
我们分析了培养的大鼠海马神经元轴突和树突中微管的极性取向。正如以前报道的来自其他神经元的轴突，这些轴突中的微管在极性上是一致的；(+)-末端从细胞体指向生长锥。与之形成鲜明对比的是，枝晶中间区域的微管。与胞体相距75µm，不具有均匀的极性取向。这些微管的方向大致相等，(+)端朝向生长锥体，(+)端朝向细胞体。然而，在距离生长锥15米的范围内，树突中的微管极性方向与轴突中的类似；(+)-末端均匀地指向生长锥。这些发现表明，轴突和树突在微管组织方面存在明显的差异，这种差异可能是神经元内细胞器分布不同的原因。
We have analyzed the polarity orientation of microtubules in the axon and dendrites of cultured rat hippocampal neurons. As previously reported of axons from other neurons, microtubules in these axons are uniform with respect to polarity; (+)-ends are directed away from the cell body towards the growth cone. In sharp contrast, microtubules in the mid-region of the dendrite, .apprxeq. 75 .mu.m from the cell body, are not of uniform polarity orientation. Roughly equal proportions of these microtubules are oriented with (+)-ends directed toward the growth cone and (+)-end directed toward the cell body. At distances within 15 .mu.m of the growth cone, however, microtubule polarity orientation in dendrites is similar to that in axons; (+)-ends are uniformly directed toward the growth cone. These findings indicate a clear difference between axons and dendrites with respect to microtubule organization, a difference that may underlie the differential distribution of organelles within the neuron.