Human induced pluripotent stem cells free of vector and transgene sequences.
Human induced pluripotent stem cells free of vector and transgene sequences.
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DOI：
10.1126/science.1172482
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发表时间：
2009-05-08
期刊：
影响因子：
--
通讯作者：
Thomson JA
中科院分区：
文献类型：
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作者：
Yu J;Hu K;Smuga-Otto K;Tian S;Stewart R;Slukvin II;Thomson JA
将分化的人类细胞重编程为诱导多能干细胞（iPS）在基础生物学、药物开发和移植等领域具有广泛的应用。人类iPS细胞的衍生以前需要整合到基因组中的载体，这可能会产生突变并限制细胞在研究和临床应用中的效用。在这里，我们描述了人类iPS细胞的衍生使用非整合episomal载体。去除片段后，获得了完全不含载体和转基因序列的iPS细胞，其增殖和发育潜力与人类胚胎干细胞（ES）相似。这些结果表明，重编程人类体细胞不需要基因组整合或外源重编程因子的持续存在，并消除了人类iPS细胞临床应用的一个障碍。
Reprogramming differentiated human cells to induced pluripotent stem (iPS) cells has applications in basic biology, drug development, and transplantation. Human iPS cell derivation previously required vectors that integrate into the genome, which can create mutations and limit the utility of the cells in both research and clinical applications. Here we describe the derivation of human iPS cells using non-integrating episomal vectors. After removal of the episome, iPS cells completely free of vector and transgene sequences are derived that are similar to human embryonic stem (ES) cells in proliferative and developmental potential. These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors, and removes one obstacle to the clinical application of human iPS cells.