Negative interference dominates collective transport of kinesin motors in the absence of load
Negative interference dominates collective transport of kinesin motors in the absence of load
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DOI：
10.1039/b900964g
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发表时间：
2009-01-01
影响因子：
3.3
通讯作者：
Diehl, Michael R.
中科院分区：
文献类型：
--
作者：
Rogers, Arthur R.;Driver, Jonathan W.;Diehl, Michael R.
已知马达蛋白的集体功能对于许多细胞内货物的定向运输是重要的。然而，了解多个电机如何作为一个群体发挥作用仍然具有挑战性，需要新的方法，使参与运动的电机的确切数量和他们的组织亚细胞货物的确定。在这里，我们提出了一种生物合成方法，使两个驱动蛋白-1分子被组织在线性支架上，分开的电机由50纳米的距离。跟踪这些复合物的运动显示，虽然两个电机产生更长的平均运行长度比单一的驱动蛋白，系统有效地表现出好像一个单一的电机附件状态占主导地位的运动。有人提出，负电机干扰来自异步电机步进和电机之间的力的通信导致这种行为，通过促进不同的微管绑定配置的组件之间的快速交换。
The collective function of motor proteins is known to be important for the directed transport of many intracellular cargos. However, understanding how multiple motors function as a group remains challenging and requires new methods that enable determination of both the exact number of motors participating in motility and their organization on subcellular cargos. Here we present a biosynthetic method that enables exactly two kinesin-1 molecules to be organized on linear scaffolds that separate the motors by a distance of 50 nm. Tracking the motions of these complexes revealed that while two motors produce longer average run lengths than single kinesins, the system effectively behaves as though a single-motor attachment state dominates motility. It is proposed that negative motor interference derived from asynchronous motor stepping and the communication of forces between motors leads to this behavior by promoting the rapid exchange between different microtubule-bound configurations of the assemblies.