Evolution of thioester-containing proteins revealed by cloning and characterization of their genes from a cnidarian sea anemone, Haliplanella lineate
Evolution of thioester-containing proteins revealed by cloning and characterization of their genes from a cnidarian sea anemone, Haliplanella lineate
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DOI：
10.1016/j.dci.2010.02.011
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发表时间：
2010-07-01
影响因子：
2.9
通讯作者：
Nonaka, Masaru
中科院分区：
文献类型：
--
作者：
Fujito, Naoko T.;Sugimoto, Sanae;Nonaka, Masaru
为了阐明编码含硫酯蛋白（TEPs）的基因的进化起源，TEP基因从刺胞动物，海葵，Haliplanella lineate分离。系统发育树分析表明，这4种刺胞动物的TEP基因和多种后生动物的TEP基因可分为两个亚家族：编码α 2-巨球蛋白（A2 M）、CD 109和昆虫TEP的A2 M亚家族和编码补体C3的C3亚家族。C4和C5。四个刺胞动物TEP基因中的两个属于A2 M亚家族，分别与人类A2 M和CD 109显示出密切的相似性，因此被称为HaliA 2 M和HaliCD 109。另外两个基因属于C3亚家族，被命名为HaliC 3 -1和HaliC 3 -2。刺胞动物TEPs保留了基本的结构域和功能上重要的残基缺口分子，其mRNA在海葵体的不同部位均有表达。这些结果表明，C3，A2 M和CD 109之间的基因复制和随后的功能分化是非常古老的事件之前的分歧刺胞动物和bilateria。(C)2010爱思唯尔有限公司版权所有。
To elucidate the evolutionary origin of genes encoding thioester-containing proteins (TEPs), TEP genes were isolated from a cnidarian, a sea anemone, Haliplanella lineate. Phylogenetic tree analysis of the four identified cnidarian TEP genes and various TEP genes of many metazoa, indicated that they could be classified into two subfamilies: the alpha-2-macroglobulin (A2M) subfamily encodining A2M, CD109 and insect TEPs, and the C3 subfamily encoding complement C3. C4 and C5. Two of the four cnidarian TEP genes belonged to the A2M subfamily, showing a close similarity to human A2M and CD109, respectively and thus were termed HaliA2M and HaliCD109. The other two genes belonged to the C3 subfamily, and were termed HaliC3-1 and HaliC3-2. Cnidarian TEPs retained the basic domain structure and functionally important residues breach molecule, and their mRNA were detected at different parts of the sea anemone body. These results suggest that gene duplication and subsequent functional differentiation among C3, A2M and CD109 were very ancient events predating the divergence of the cnidaria and bilateria. (C) 2010 Elsevier Ltd. All rights reserved.