PROTEIN-PHOSPHORYLATION IS INVOLVED IN BACTERIAL CHEMOTAXIS
PROTEIN-PHOSPHORYLATION IS INVOLVED IN BACTERIAL CHEMOTAXIS
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DOI：
10.1073/pnas.84.21.7609
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发表时间：
1987-11-01
影响因子：
11.1
通讯作者：
SIMON, MI
中科院分区：
文献类型：
--
作者：
HESS, JF;OOSAWA, K;SIMON, MI
在细菌趋化性中参与激发反应的生化信号的性质尚不清楚。然而，ATP是趋化性所必需的。我们已经纯化了所有参与信号转导的蛋白质，并表明cheA基因的产物迅速自磷酸化，而一些突变的CheA蛋白不能磷酸化。化学计量水平的存在下，其他两个纯化的组分在趋化系统中，CheY和CheZ蛋白，诱导去磷酸化。我们认为ATP对CheA的磷酸化在趋化性的信号转导中起着重要作用。
The nature of the biochemcial signal that is involved in the excitation response in bacterial chemotaxis is not known. However, ATP is required for chemotaxis. We have purified all of the proteins involved in signal transduction and show that the product of the cheA gene is rapidly autophosphorylated, while some mutant CheA proteins cannot be phosphorylated. The presence of stoichiometric levels of two other purified components in the chemotaxis system, the CheY and CheZ proteins, induces dephosphorylation. We suggest that the phosphorylation of CheA by ATP plays a central role in signal transduction in chemotaxis.