Interactions between the omega- and beta-oxidations of fatty acids.
Interactions between the omega- and beta-oxidations of fatty acids.
复制标题
脂肪酸的欧米伽氧化和β氧化之间的相互作用。
DOI：
10.1093/oxfordjournals.jbchem.a122035
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发表时间：
1987
影响因子：
2.7
通讯作者：
Draye,JP
中科院分区：
文献类型：
--
作者：
Vamecq,J;Draye,JP
长链单羧酸、ω-羟基单羧酸和二羧酸被大鼠肝匀浆以近似相同的速率活化成其CoA酯（2- 3U/g肝）。这些酰基辅酶A是大鼠肝脏过氧化物酶体β-氧化的底物。还研究了这些底物的过氧化物酶体氧化在各种组织中的分布。大鼠肝线粒体能够氧化长链单羧基和ω-羟基单羧基辅酶A，但不能氧化二羧基辅酶A。当线粒体制剂在偶联条件下孵育时，加入游离癸酸或游离10-羟基癸酸导致氧摄取增加，与加入癸二酸相反。对过氧化物酶体脂肪酰辅酶A氧化酶和线粒体脂肪酰辅酶A脱氢酶活性的链长底物特异性的比较研究表明，实际上，过氧化物酶体和线粒体这两种类型的细胞器都含有对长链单羧基辅酶A、ω-羟基-单羧基辅酶A和二羧基辅酶A有活性的“氧化还原酶”。
Long-chain monocarboxylic, ω-hydroxymonocar boxy lie and dicarboxylic acids were activated approximatively at the same rate by rat liver homogenates into their CoA esters (2–3 U/g liver). These acyl-CoA were substrates for rat liver peroxisomal β-oxidation. The distribution of the peroxisomal oxidation of these substrates was also studied in various tissues. Rat liver mitochondria were capable of oxidizing long-chain monocarboxyl- and ω-hydroxymonocarboxylyl-CoAs but not dicar-boxylyl-CoAs. When the mitochondrial preparations were incubated in coupling conditions, the addition of either free decanoic acid or free 10-hydroxydecanoic acid resulted in an increase of the oxygen uptake conversely to the addition of decanedioic acid. The comparative study of the chain-length substrate specificity of peroxisomal fatty acyl-CoA oxidase and mitochondrial fatty acyl-CoA dehydrogenase activities revealed that, actually, both types of organelles, peroxisomes and mitochondria, contain “oxido-reductases” active on long-chain monocarboxylyl-CoAs, ω-hydroxy-monocarboxylyl-CoAs and dicarboxylyl-CoAs.