Colwellia marinimaniae sp. nov., a hyperpiezophilic species isolated from an amphipod within the Challenger Deep, Mariana Trench.
Colwellia marinimaniae sp. nov., a hyperpiezophilic species isolated from an amphipod within the Challenger Deep, Mariana Trench.
复制标题
DOI：
10.1099/ijsem.0.001671
复制
发表时间：
2017-04
影响因子：
2.8
通讯作者：
M. Kusube;Than S. Kyaw;Kumiko Tanikawa;R. Chastain;Kevin Hardy;James Cameron;D. Bartlett
中科院分区：
文献类型：
--
作者：
M. Kusube;Than S. Kyaw;Kumiko Tanikawa;R. Chastain;Kevin Hardy;James Cameron;D. Bartlett
在 DEEPSEA CHALLENGE 探险期间，从马里亚纳海沟挑战者深海地区获得的片足类甲壳类动物中分离出专性亲压菌株。该菌株 MTCD1T 在极高的静水压力下生长，6°C 时的生长范围为 80-140 MPa（最佳为 120 MPa）。基于16S rRNA基因序列的系统发育分析表明它与Colwellia属密切相关。比较 16S rRNA 基因序列分析显示与 Colwellia maris ABE-1T、Colwellia piezophila Y233GT 和 Colwellia psychrerythraea ATCC 27364T 的相似性分别为 95.7%、95.5% 和 95.2%。主要的细胞脂肪酸是C16:1、C16:0和C22:6（二十二碳六烯酸），唯一产生的类异戊二烯醌是ubiqinone-8。 DNA G+C含量为48.6mol%。该菌株的氧化酶和过氧化氢酶活性呈阳性。根据本研究的结果，菌株MTCD1T是Colwellia属的一种新的革兰氏阴性菌，名称为Colwellia marinimaniae sp。十一月提出了（类型菌株 MTCD1T=ATCC TSD-5T=JCM 30270T）。它是迄今为止描述的最亲压的生物体。
An obligately piezophilic strain was isolated from an amphipod crustacean obtained in the Challenger Deep region of the Mariana Trench during the DEEPSEA CHALLENGE expedition. The strain, MTCD1T, grew at extremely high hydrostatic pressures, with a growth range of 80-140 MPa (optimum, 120 MPa) at 6 °C. Phylogenetic analyses based on the 16S rRNA gene sequence indicate that it is closely affiliated with the genus Colwellia. Comparative 16S rRNA gene sequence analyses revealed 95.7, 95.5 and 95.2 % similarity to Colwellia maris ABE-1T, Colwellia piezophila Y233GT and Colwellia psychrerythraea ATCC 27364T, respectively. The major cellular fatty acids were C16 : 1, C16 : 0 and C22 : 6 (docosahexaenoic acid), and the sole isoprenoid quinone produced was ubiqinone-8. DNA G+C content was 48.6 mol%. The strain was positive for oxidase and catalase activities. Based on the results from this study, strain MTCD1T is a novel Gram-negative species of the genus Colwellia, and the name Colwellia marinimaniae sp. nov. (type strain MTCD1T=ATCC TSD-5T=JCM 30270T) is proposed. It is the most piezophilic organism yet described.