Immunohistochemical distribution of inter-α-trypsin inhibitor chains in normal and malignant human lung tissue
Immunohistochemical distribution of inter-α-trypsin inhibitor chains in normal and malignant human lung tissue
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DOI：
10.1177/002215549904701214
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发表时间：
1999-12-01
影响因子：
3.2
通讯作者：
Thiberville, L
中科院分区：
文献类型：
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作者：
Bourguignon, J;Borghi, H;Thiberville, L
间 α E E-胰蛋白酶抑制剂 (ITI) 家族是一组血浆蛋白，由肝脏中合成的重链（HC1、HC2、HC3）和紧链（bikunin）组成。在这项研究中，我们使用多克隆抗体确定了正常和癌性肺组织中 ITI 组成链的分布。在正常肺组织中，HZ、H3和bikunin链存在于多形核细胞中，而H1和bikunin蛋白存在于肥大细胞中。在支气管上皮粘液细胞中进一步观察到 Bikunin。在肺癌中，在肿瘤胰岛周围的浸润性多形核细胞和肥大细胞上也获得了类似的发现。在腺癌和鳞状细胞癌中，高度分化的癌细胞均显示出 H1 和 Bikunin 抗血清的强胞质内染色。此外，在腺癌细胞中观察到微弱但频繁的HZ表达，而在癌细胞中未检测到H3相关蛋白。通过RT-PCR证实局部肺ITI表达。尽管目前还不能阐明炎症细胞和肿瘤细胞在ITI链合成中各自的作用，但这些结果表明重链和bikunin都参与肺组织的恶性转化。
The inter-alpha E E-trypsin inhibitor (ITI) family is a group of plasma proteins built up from heavy (HC1, HC2, HC3) and tight (bikunin) chains synthesized in the liver. In this study we determined the distribution of ITI constitutive chains in normal and cancerous lung tissues using polyclonal antibodies. In normal lung tissue, HZ, H3, and bikunin chains were found in polymorphonuclear cells, whereas H1 and bikunin proteins were found in mast cells. Bikunin was further observed in bronchoepithetial mucous cells. In lung carcinoma, similar findings were obtained on infiltrating polymorphonuclear and mast cells surrounding the tumor islets. Highly differentiated cancerous cells displayed strong intracytoplasmic staining with H1 and bikunin antiserum in both adenocarcinoma and squamous cell carcinoma. Moreover, weak but frequent HZ expression was observed in adenocarcinoma cells, whereas no H3-related protein could be detected in cancer cells. Local lung ITI expression was confirmed by RT-PCR. Although the respective role of inflammatory and tumor cells in ITI chain synthesis cannot be presently clarified, these results show that heavy chains as well as bikunin are involved in malignant transformation of lung tissue.