Angiogenesis in mucous retention cyst: a human in vivo-like model of endothelial cell differentiation in mucous substrate.
Angiogenesis in mucous retention cyst: a human in vivo-like model of endothelial cell differentiation in mucous substrate.
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DOI：
10.1111/j.1600-0714.2004.00282.x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
W. Swelam;H. Ida-Yonemochi;T. Saku
中科院分区：
文献类型：
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作者：
W. Swelam;H. Ida-Yonemochi;T. Saku
背景：粘液潴留囊肿在腔内含有粘液池，其中偶尔观察到纯粹的血管生成过程。通过使用这种独特的人体材料，我们的目标是了解体内血管生成过程。方法采用免疫组织化学和原位杂交（ISH）技术检测15例唇部粘膜保留囊肿手术组织标本中血管内皮标志物和细胞外基质分子的表达。结果形成新血管通道的内皮细胞CD31、CD34、血管内皮生长因子（VEGF）、血管性血液病因子（vWF）免疫阳性。这些新形成的毛细血管被tenascin阳性基质包围，并进一步被cd68阳性细胞密集浸润，具有泡沫样上皮外观。其中一些细胞同时对CD34、VEGF及其受体之一Flk-1呈阳性，并且它们显示出明确的腱素mRNA和蛋白信号。此外，这些细胞往往倾向于排列，这表明小管形成。结论单核/巨噬细胞系细胞是内皮细胞的主要来源，至少在粘膜保留囊肿中是如此，这些细胞产生的腱素在内皮细胞的分化中起重要作用。
BACKGROUND Mucous retention cysts contain a mucous pool in the lumina, in which pure angiogenic processes are occasionally observed. By using this unique human material, our aim was to understand the in vivo angiogenic process. METHODS Fifteen surgical tissue samples of mucous retention cysts of the lip were examined for expression of vascular endothelial markers and extracellular matrix molecules by immunohistochemistry and in situ hybridization (ISH). RESULTS Endothelial cells forming new vascular channels showed immunopositivities for CD31, CD34, vascular endothelial growth factor (VEGF), and von Willebrand factor (vWF). These newly formed capillaries were surrounded by tenascin-positive matrices and further by a dense infiltration of CD68-positive cells with foamy to epitheloid appearances. Some of these cells were simultaneously positive for CD34, VEGF, and one of its receptors, Flk-1, and they showed definite mRNA as well as protein signals for tenascin. In addition, these cells often tended to be aligned, which suggested tubule formation. CONCLUSION The results suggest that monocyte/macrophage lineage cells are a major source for endothelial cells at least in mucous retention cysts and that tenascin produced by those cells plays an important role in differentiation of endothelial cells.