Phosphatidylcholine and lysophosphatidylcholine in intestinal mucus of ulcerative colitis patients. A quantitative approach by nanoelectrospray-tandem mass spectrometry
Phosphatidylcholine and lysophosphatidylcholine in intestinal mucus of ulcerative colitis patients. A quantitative approach by nanoelectrospray-tandem mass spectrometry
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DOI：
10.1080/00365520410006233
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发表时间：
2004-08-01
影响因子：
1.9
通讯作者：
Stremmel, W
中科院分区：
文献类型：
--
作者：
Ehehalt, R;Wagenblast, J;Stremmel, W
背景：粘液组成缺陷是肠道损伤的关键致病因素。磷脂酰胆碱（PC）是促成疏水黏液层形成的重要成分。为了评估PC在炎症性肠病发病机制中的作用，我们测定了溃疡性结肠炎患者直肠黏液中PC的浓度和组成。电喷雾电离（ESI）串联质谱（MS/MS）可以定量PC物种，并可以分析粗提取物。方法：采用纳米esi质谱联用技术，以合成内质谱和溶血磷脂酰胆碱（LPC）为标准，定量分析光刮法获得的肠腔脂质提取物。将溃疡性结肠炎患者直肠镜下获得性黏液中PC和LPC种类与克罗恩病和对照组进行比较。结果：与克罗恩病（1126 [IQR: 4651-9411 pmol总PC/mg干重）和对照组（1285 [IQR: 850-1639] pmol总PC/mg干重）相比，非活动性溃疡性结肠炎患者直肠黏液中PC和LPC（中位数为346 [IQR: 2304051 pmol总PC/mg干重）显著减少（P < 0.05）。各组间PC和LPC的分子种类差异不显著。最丰富的种数为PC 16:0/18:1；PC 16:0/18:2;， PC 18:0/18:1；PC 18:0/18:2 LPC 16:0：和LPC 18:0。结论：纳米esi质谱法是测定人黏液中微量PC的有效方法。溃疡性结肠炎患者的肠黏液中PC显著减少，尽管PC分子种类组成模式相似。这表明保护性黏液PC含量低是溃疡性结肠炎的特征，并解释了对肠管内容物的易感性增加。
Background: A defective mucus composition represents a key pathogenetic factor for intestinal injury. Phosphatidylcholine (PC) is an essential component contributing to formation of a hydrophobic mucus layer. For evaluation of PC in the pathogenesis of inflammatory bowel disease, the concentration and composition of PC in the rectal mucus Of patients with ulcerative colitis was determined. Electrospray ionization (ESI) tandem mass spectrometry (MS/MS) allows quantification of PC species and enables analysis of crude extracts. Methods: Lipid extracts of material obtained by light scrapings of the intestinal lumen were analysed quantitatively by nanoESI MS/MS with synthetic internal PC and lysophosphatidylcholine (LPC) standards. PC and LPC species from rectoscopically acquired Mucus aliquots of patients with ulcerative colitis were compared to Crohn disease and control subjects. Results: Patients with inactive ulcerative colitis showed significantly less PC and LPC (median 346 [IQR: 2304051 pmol total PC/mg dry weight) in rectal mucus compared to Crohn disease (median 1126 [IQR: 4651-9411 pmol total PC/mg dry weight) and control subjects (median 1285 [IQR: 850-1639] pmol total PC/mg dry weight) (P < 0.05). The molecular species of PC and LPC were not significantly different between the groups. The most abundant species were PC 16:0/18:1; PC 16:0/18:2;, PC 18:0/18:1; PC 18:0/18:2 LPC 16:0: and LPC 18:0. Conclusion: NanoESI MS/MS is a suitable tool for analysing and quantifying small amounts of PC in human mucus. Patients with ulcerative colitis have significant less PC in their intestinal mucus despite a comparable PC molecular species composition pattern. This suggests that a low amount of protective mucus PC is a characteristic feature in ulcerative colitis and explains an increased susceptibility to luminal contents.