A new in vitro toxicity test based on the response to toxic substances in solutions of mitochondria from beef heart
A new in vitro toxicity test based on the response to toxic substances in solutions of mitochondria from beef heart
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基于对牛心线粒体溶液中有毒物质的反应的新体外毒性测试
DOI：
10.1007/bf00213179
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发表时间：
1994
影响因子：
4
通讯作者：
P. Dell'Antone
中科院分区：
文献类型：
--
作者：
M. Bragadin;P. Dell'Antone
本文提出了一种简便、快速、廉价的体外方法，可作为评价水中化合物毒性的生物传感器。该方法利用牛心线粒体呼吸链对多种有毒物质的反应。通过简单的伏安测量，该方法不仅给出了水中毒性的指示，而且通过使用呼吸链的不同底物和利用有毒物质的某些特征，它还允许选择性地检测某些有毒物质或物质组。由于该方法再现了标准的“虹鳟鱼”测试，它也可以被提议作为一个筛选测试之前，整个生物有机体的生物测定或作为一种方法来评估某些有毒物质在水中的存在。
A simple, rapid and inexpensivein vitromethod which may be used as a biological sensor for the valuation of toxicity of chemical compounds in water is proposed. The method utilizes the response of the respiratory chain of beef heart mitochondria to a wide range of toxic substances. By means of simple voltametric measurements, the method not only gives an indication of toxicity in water but, by using different substrates of the respiratory chain and exploiting some features of the toxic substances, it also allows selective detection of some toxic substance or groups of substances. Since the method reproduces the standard “rainbow trout” test, it may also be proposed as a screening test to precede whole bioorganisms bioassays or as a method to assess the presence of certain toxic substances in water.